Fruit tree multi-provenance quality seed breeding method
A kind of fruit tree variety and fruit tree technology, applied in the field of multi-source quality breeding of fruit trees, can solve the problems of extinction, the brand value of famous product varieties cannot be effectively utilized, and resource destruction
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[0086] The preparation method of the macroelement mother solution of MS basic medium: mix 1650mgNH 4 NO 3 , 1900mgKNO 3 , 440mgCaCl 2 2H 2 O, 370mgMgSO 4 ·7H 2 O and 170 mg KH 2 PO 4 Dissolve in water and make up to 1L with water.
[0087] Seedling-raising substrate: The seedling-raising substrate adopted in the embodiments of the present invention is the plug substrate used for planting flower seedlings produced by Shandong Yijing Garden Co., Ltd., which is generally produced and sold by various flower seedling companies.
Embodiment 1
[0088] Embodiment 1, apple breeding
[0089] 1. Preparation of mixed pollen
[0090] (1) Preparation of pollen
[0091] 1. Hybridization (carried out in Luntai County, Xinjiang)
[0092] In April 2006, the pollen of 'Jinshuai' apple was used to pollinate the emasculated 'Tal Alma' apple, and the F 1 hybrid seed, the F 1 After the hybrid seeds are washed, they are placed in a fresh-keeping room of a refrigerator at 1-3°C for stratification treatment for about 60 days (the purpose is to release the dormancy of the seeds by satisfying the required cooling capacity).
[0093] 2. Seedling cultivation in greenhouse
[0094] In December, 2006, the seeds that completed step 1 were sown in a nutrient pot (3-5 seeds were planted in each nutrient pot) that completed step 1 and cultivated to a seedling height of 8-15 cm and lignification at the root neck (usually 2-3 months after sowing), and then the seedlings are transplanted to a new nutrient pot (one plant is transplanted in each...
Embodiment 2
[0154] Embodiment 2, pear breeding
[0155] 1. Hybridization (carried out in Tarim, Xinjiang)
[0156] In April 2003, the pollen of ‘Laiyangci’ pear was used to pollinate the emasculated ‘Xinli No. 7’ pear, and the F 1 hybrid seed, the F 1 After the hybrid seeds are washed, they are placed in a fresh-keeping room of a refrigerator at 1-3°C for stratification treatment for about 60 days (the purpose is to release the dormancy of the seeds by satisfying the required cooling capacity).
[0157] 2. Greenhouse seedling cultivation (in Tai'an, Shandong)
[0158] In December 2003, the F obtained in step 1 1 The hybrid seeds are sown in a nutrient pot (3-5 seeds are planted in each nutrient pot) that is equipped with a seedling-raising substrate and cultivated until the seedling height is 8-15cm and the root neck is lignified (usually 2-3 months for sowing), and then The seedlings are transplanted to new nutrient pots (every nutrient pots are transplanted with 1 plant) that are eq...
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