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Genetic locus rs780092 and assay kit for type-2 diabetes disease risk assessment

A type 2 diabetes and kit technology, applied in the field of genetic locus rs780092 and detection kits, can solve the differences in genetic basis and living environment between Chinese and Western populations, the accuracy lacks large-scale prospective studies, and has not been included in the risk assessment system and other issues, to achieve the effect of economy and convenience

Inactive Publication Date: 2015-12-02
CAPITALBIO CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Specifically, the existing risk assessment methods for type 2 diabetes have the following defects: First, the influence of blood lipid-related genetic loci on the risk of type 2 diabetes is still unclear and has not been included in the risk assessment system
Therefore, existing methods for assessing type 2 diabetes risk are not individualized enough
Third, although there are relevant research results abroad, there are obvious differences in the genetic basis and living environment of Chinese and Western populations, and the accuracy of foreign research results in Chinese populations lacks large-scale prospective research verification

Method used

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  • Genetic locus rs780092 and assay kit for type-2 diabetes disease risk assessment
  • Genetic locus rs780092 and assay kit for type-2 diabetes disease risk assessment
  • Genetic locus rs780092 and assay kit for type-2 diabetes disease risk assessment

Examples

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Embodiment 1

[0035] Example 1. Association analysis between the single nucleotide polymorphism of rs780092 site and the risk of developing type 2 diabetes

[0036] 1. Preparation of detection kits for risk assessment of blood lipids and type 2 diabetes

[0037] 1. Kit composition

[0038] The detection kit for blood lipid and type 2 diabetes risk assessment developed by the inventors of the present invention includes a PCR primer pair for amplifying the gene fragment at the rs780092 site, and a SequenomMassARRAY single-base extension primer for specifically detecting the genotype at the rs780092 site . The sequences of the PCR primer pair are shown as sequence 1 (SEQ ID NO.1) and sequence 2 (SEQ ID NO.2) in the sequence listing. The sequence of the single base extension primer is shown as sequence 3 (SEQ ID NO.3) in the sequence listing.

[0039] SEQ ID NO.1: 5'-ACGTTGGATGCCTAGCTCAAAATACCACCC-3';

[0040] SEQ ID NO.2: 5'-ACGTTGGATGGGAGGTATAAAGAAGGTGGG-3';

[0041] SEQ ID NO. 3: 5'-TTC...

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Abstract

The invention discloses an assay kit for type-2 diabetes disease risk assessment on the basis of GCKR genetic locus rs780092, and further provides an application of substance for testing single nucleotide polymorphism of the genetic locus rs780092 in preparing products for assessing or assisting in assessing type-2 diabetes disease risk for people under test. According to the invention, influence of the genetic locus rs780092 related to blood lipid to type-2 diabetes disease risk is cleared and defined and is brought into a risk assessment system; on the basis of the genetic locus rs780092 related to blood lipid, influence to type-2 diabetes disease risk of different individuals caused by rising of blood lipid is assessed, and factors of type-2 diabetes disease risk assessment adaptable to Chinese people are researched in large-scale prospective cohort. The assay kit and the application have great significance in solving problems about comprehension, individuation and accuracy of type-2 diabetes disease risk assessment.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a genetic locus rs780092 and a detection kit for assessing the risk of developing type 2 diabetes. Background technique [0002] The prevalence of diabetes in my country is showing a rapid upward trend, and it has become another important chronic non-communicable disease that seriously endangers people's health after cardiovascular and cerebrovascular diseases and tumors. The results of a large-scale epidemiological survey published in JAMA, the Journal of the American Medical Association in September 2013 showed that the prevalence of diabetes in people over the age of 18 in my country was 11.6%, which has become the country with the largest number of diabetic patients in the world. Among them, type 2 diabetes accounts for more than 90%. At present, my country has entered a period of high burden of chronic diseases, with the characteristics of "large number of patients, high medical co...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 孙义民宁光周禹稀徐敏
Owner CAPITALBIO CORP
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