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Method for doubling young embryos of maize haploids

A haploid immature embryo and haploid technology, applied in the biological field, can solve the problems of high consumption of chemicals, insufficient efficiency of haploid doubling, and high treatment concentration

Inactive Publication Date: 2015-12-02
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are mainly the following disadvantages: (1) the doubling of haploids is still not efficient enough, especially the doubling of tassels; (2) haploid selection depends on the color of the induced line, because the color expression is a process of gradual accumulation, in order to be able to Accurate selection needs to be selected at a later developmental stage, but in the late stage of development, the embryo is already at a higher developmental stage and it is not conducive to doubling, so high-concentration medicaments are required for doubling to ensure doubling efficiency. The treatment concentration of medicaments is high, Not only consumes a lot of chemicals, but also harms the seedlings, handlers and the environment

Method used

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  • Method for doubling young embryos of maize haploids

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preparation example Construction

[0030] Preparation method of MS medium: Each liter of MS medium contains 3.0 g of MS salt (MS salt is a product of Shanghai Yuhan Biotechnology Co., Ltd., product catalog number 140225), 30 g of sucrose, and dilute to 1 L after dissolving. When the pH value reaches 5.8, pour it into a conical flask and add 7.5g of agar.

[0031] The preparation method of the MS medium containing the doubling agent: add an appropriate amount of doubling agent and auxiliary agent (Tween and dimethyl sulfoxide) to the MS medium, and mix well to obtain the MS medium containing the doubling agent. Wherein, doubling medicament: Amamidophos-methyl (APM) is the product of sigma company, and the product catalog number is Sigma03992; Tween 80: add after separately sterilizing or directly add medium sterilization all can; Dimethyl sulfoxide ( DMSO): added after filter sterilization.

Embodiment 1

[0032] Embodiment 1, the method for doubling of corn haploid immature embryos

[0033] (1) Screening the optimum doubling reagent concentration for doubling of maize haploid immature embryos from the seedling rate

[0034] 1. Obtaining immature embryos of hybrid offspring

[0035] 1. Hybridization

[0036] (1) Hybrid parent

[0037] Taking Zhengdan 958 as the female parent, the fluorescence inducible line is CAU YFP As the male parent, hybridize to obtain hybrid offspring.

[0038] (2) Specific method of hybridization

[0039] Before the filaments of the female parent are spit out, they are detasseled and the ears are bagged; after the filaments are spit out, the filaments are cut at the same time, and the pollination is uniform on the next day. About 12 days after pollination, a hybrid with an embryo length of 2.0-3.0mm is obtained. Offspring;

[0040] At the same time, the first selfed generation of Zhengdan 958 was used as the control progeny for fluorescence observat...

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Abstract

The invention discloses a method for doubling young embryos of maize haploids. The method disclosed by the invention comprises the following step: doubling young embryos of maize haploids in a system containing 0.5-1.0 [mu]mol / L amiprophos-methyl to double the young embryos of maize haploids. The method disclosed by the invention can be used for remarkably improving the doubling efficiency of the maize haploids, and the tassel powder rate is near 30%. Moreover, the method is relatively less in damage to the environmental pollution and seedlings, and the plants grow relatively well, thereby providing a support for improving the breeding efficiency of the haploids.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for doubling corn haploid immature embryos. Background technique [0002] Maize is a model crop that utilizes heterosis, and the most critical link is pure line selection. The breeding of conventional maize inbred lines requires multiple generations of self-selection, so it often takes several years to breed a variety; and theoretically, even if self-crossing n(n∝∞) generations, the genotype cannot 100% complete homozygosity is achieved. Using the parthenogenetic induction line to induce haploids and artificially double, only two generations can be used to obtain 100% homozygous inbred lines. Compared with conventional methods, it not only greatly shortens the breeding process, but also saves a lot of manpower and material resources. and financial resources. [0003] Common methods of doubling haploids include natural doubling and chemical doubling. The frequ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H1/04
Inventor 陈绍江陈宝建刘丽威孟玉杰
Owner CHINA AGRI UNIV
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