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Temperature-controlled grafting method for regenerated plants of transgenic cotton

A technology of warm grafting method and regenerated plant, applied in the field of temperature-controlled grafting method of transgenic cotton regenerated plant, can solve problems such as failure to achieve, and achieve the effect of improving the survival rate and reducing the death of the whole plant

Inactive Publication Date: 2015-12-02
COTTON RES INST SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to solve the above existing problems, a series of transgenic cotton grafting methods have been developed at present (patent announcement numbers are respectively CN201595049U, CN202340415U, CN102893813A), although the survival rate of grafted seedlings has been improved to a certain extent, it still cannot reach intended purpose

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] A method for temperature-controlled grafting of regenerated transgenic cotton plants, which specifically includes the following operating procedures:

[0011] Step 1. Preparation of rootstock seedlings: select sea-island cotton seeds with good disease resistance and long growth period to sow in small pots (10-12 cm high, 10-12 cm in diameter at the upper opening, and 9 cm in diameter at the lower opening), and the seedlings will emerge for 1-4 cm. One true leaf is ready for use;

[0012] Step 2. Grafting and temperature control: (1) The transgenic regenerated plants are grafted to the rootstock of the pot and tied with a rope; (2) The grafted seedlings of the pot are fixed with iron wires, and the periphery of the grafted seedlings is wrapped with a plastic film to the pot. (3) Tighten the wrapped plastic film, and insert the head into the plastic film; (4) Put the grafted seedling pot in a place where the temperature of the environment space is ≤28°C; (5) Release the s...

Embodiment 2

[0014] A method of temperature-controlled grafting of transgenic cotton regenerated plants, during high temperature periods in summer (atmospheric temperature ≥ 35°C), grafting cotton transgenic regenerated plants in small pots to grow islands with 1 to 4 true leaves that have good disease resistance and a long growth period On cotton rootstocks, wrap the grafted seedling pots with plastic film and place the grafted seedling pots in a place where the ambient space temperature is ≤28°C. The space temperature of the transgenic regenerated plants will be lowered, and the root of the rootstock will not rot and cause the death of the entire plant. Part of the grafted seedlings did not rot and die. Therefore, the transplantation survival rate of the transgenic regenerated strain is very high, so this is a simple and ideal method for transplanting the transgenic regenerated strain.

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Abstract

The invention discloses a temperature-controlled grafting method for regenerated plants of transgenic cotton. The method comprises the following specific operation steps: 1, preparation of stock seedlings, wherein gossypium barbadense seeds which are good in disease resistance and long in growing period are selected and sowed in a small basin, the height of the small basin is 10-12 cm, the diameter of an upper opening is 10-12 cm, the diameter of a lower opening is 9 cm, and when 1-4 main leaves emerge, the seedlings are reserved for standby application; 2, grafting and temperature control. By means of the method, the phenomenon that the total plants die due to the fact that at the high temperature time in summer, stock roots or the upper portions of the regenerated plants of the transgenic cotton are prone to being rotten can be effectively reduced, when the atmosphere temperature is equal to or greater than 35 DEG C in summer, after the grafted seedlings are grafted and 1-7 days later, and when the wrapped space temperature is less than or equal to 28 DEG C, the survival rate of the grafted seedlings can reach over 95%, and the survival rate of the regenerated plants of the transgenic cotton can be raised by over 15% in a high temperature season.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for temperature-controlled grafting of regenerated transgenic cotton plants. Background technique [0002] At present, the conventional transplantation method of transgenic cotton regenerated strains is the grafting method. First, the test tube cover of the transgenic cotton regenerated strains is opened, and the seedlings are hardened in a bottle for 1-2 days, and then the transgenic cotton regenerated strains are grafted in a pot in a greenhouse or a net room. On the (cotton) rootstock planted inside, seal the whole cotton seedling with plastic film and pot together for 7-8 days, then gradually let the air out, and finally remove the plastic film. However, the roots of the rootstock or the upper part of the grafted seedlings of the regenerated transgenic cotton plants are perishable during the high temperature period in summer and cause the death of the whole plant, so the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/06
CPCA01G2/30
Inventor 马燕斌张树伟吴霞王新胜李燕娥韩青龙张相斌
Owner COTTON RES INST SHANXI ACAD OF AGRI SCI
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