Extraction method of psyllium seed polysaccharide

A technology of psyllium polysaccharide and extraction method, applied in the field of biological extraction, can solve the problems of different effects, different components of psyllium polysaccharide, etc.

Active Publication Date: 2018-01-16
陕西灏霖生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As we all know, with different extraction methods, the obtained psyllium polysaccharides have different components, and the final effects are also different.

Method used

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  • Extraction method of psyllium seed polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Semen psyllium is broken, add water 20 times its weight, reflux extraction 2 times, extract 1 hour each time, filter, and collect the filtrate for subsequent use;

[0017] 2) Concentrate the filtrate to an extract with a relative density of 1.1 at 60°C, add 90v% ethanol until the alcohol content in the mixed solution is 80v%, and centrifuge to obtain a precipitate;

[0018] 3) Wash the precipitate described in step 2) sequentially with 2 times the weight of absolute ethanol and acetone respectively, dissolve the washed precipitate with 2 times the weight of distilled water, and add 1% of the weight of the washed precipitate to enzymatically digest it with protease for 1 h , inactivate the enzyme to obtain the enzymolysis solution;

[0019] 4) Dialyze the enzymolysis solution for the first time with a dialysis bag with a molecular weight of 80,000 for 24 hours, take the retentate and carry out the second dialysis with a dialysis bag with a molecular weight of 1,500,00...

Embodiment 2

[0022] 1) Semen psyllium is broken, add water 30 times its weight, reflux extraction 3 times, extract 3h each time, filter, and collect the filtrate for subsequent use;

[0023] 2) Concentrate the filtrate to an extract with a relative density of 1.3 at 60°C, add 95v% ethanol until the alcohol content in the mixed solution is 85v%, and centrifuge to obtain a precipitate;

[0024] 3) Wash the precipitate described in step 2) sequentially with 4 times the weight of absolute ethanol and acetone, dissolve the washed precipitate with 4 times the weight of distilled water, and add 2% protease to the washed precipitate for enzymatic hydrolysis for 3 hours , inactivate the enzyme to obtain the enzymolysis solution;

[0025] 4) Carry out the first dialysis of the enzymatic solution with a dialysis bag with a molecular weight of 200,000 for 72 hours, take the retentate and carry out the second dialysis with a dialysis bag with a molecular weight of 2,000,000 for 72 hours, take the secon...

Embodiment 3

[0028] 1) Semen psyllium is broken, add water 25 times its weight, reflux extraction 2 times, extract 2 hours each time, filter, and collect the filtrate for subsequent use;

[0029] 2) Concentrate the filtrate to an extract with a relative density of 1.2 at 60°C, add 95v% ethanol until the alcohol content in the mixed solution is 85v%, and centrifuge to obtain a precipitate;

[0030] 3) Wash the precipitate described in step 2) sequentially with 3 times the weight of absolute ethanol and acetone, dissolve the washed precipitate with 3 times the weight of distilled water, and add 1.5% protease to the washed precipitate for enzymatic hydrolysis for 2 hours , inactivate the enzyme to obtain the enzymolysis solution;

[0031] 4) Dialyze the enzymatic solution for the first time with a dialysis bag with a molecular weight of 100,000 for 48 hours, take the retentate and use a dialysis bag with a molecular weight of 1,800,000 for the second dialysis for 48 hours, take the second exu...

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Abstract

The invention discloses a method for extracting plantago polysaccharides, which comprises the following steps: 1) extracting the plantago with water; 2) concentrating the water extract and ethanol precipitation to obtain a precipitate; 3) sequentially washing the precipitate with absolute ethanol, Wash with acetone separately, redissolve in distilled water, add protease for enzymolysis to obtain an enzymolysis solution; 4) carry out the first dialysis of the enzymolysis solution through a dialysis bag with a molecular weight of 80,000-200,000 for 24-72 hours, and take the retained solution through a dialysis bag with a molecular weight of 1,500,000 ~2000000 dialysis bags are subjected to second dialysis for 24 to 72 hours, the second exudate is taken and concentrated, precipitated with ethanol, and centrifuged to obtain the precipitate; 5) The precipitate described in step 4) is washed with absolute ethanol and acetone respectively, Redissolve in distilled water, add activated carbon to decolorize, filter, and spray dry to obtain the psyllium seed polysaccharide. The prepared psyllium seed polysaccharide has significant immune enhancing effect.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a method for extracting plantago polysaccharide. Background technique [0002] Semen Plantago is the dry mature seed of Plantago asiatica L. or Plantago depressaWilld., which has the effects of diuresis, clearing away heat, improving eyesight and eliminating phlegm. Dysentery, cough with profuse phlegm. Studies have shown that psyllium polysaccharide is a heteropolysaccharide composed of xylose, arabinose, rhamnose, galactose, glucose, galacturonic acid, etc. The polysaccharide is a partially fermentable dietary fiber with a molecular weight of several thousand to It can range from millions to millions. As we all know, with different extraction methods, the obtained psyllium polysaccharides have different components and ultimately different effects. The extracting process of present conventional Semen Plantago polysaccharide comprises the following st...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P37/04
Inventor 文继承
Owner 陕西灏霖生物科技有限公司
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