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Application of Turrapubin D in preparation of liver-protecting drug

A technology of turrapubind, 1. turrapubind is applied in the application field of Turrapubin D in the preparation of hepatoprotective drugs, and can solve problems such as no reports of compound activity yet

Inactive Publication Date: 2015-11-18
林天样
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There is no report on the activity of this compound

Method used

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  • Application of Turrapubin D in preparation of liver-protecting drug
  • Application of Turrapubin D in preparation of liver-protecting drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1: Isolation, preparation and structure confirmation of TurrapubinD

[0010] The preparation method of TurrapubinD is the same as that reported in the literature (Bioactive Limonoid and Triterpenoid Constituents of Turraea pubescens, J. Nat. Prod., 2013, 76, 1166-1174).

[0011] Structural confirmation: According to HRESI-MS, the molecular formula is C 29 h 36 o 8 , with an unsaturation of 12. H NMR spectrum data δ H (ppm, DMSO-d 6 , 500MHz): H-1 (4.12, t, J=5.7), H-2 (2.85, dd, J=14.9, 5.7), H-2 (2.70, dd, J=14.9, 5.7), H-5 (2.63, dd, J=7.5, 3.6), H-6 (2.49, m), H-9 (3.38, d, J=8.7), H-11 (4.35, t, J=8.7), H-12 (5.47, d, J=8.7), H-15 (3.87, s), H-16 (1.80, m), H-16 (2.24, dd, J=14.0, 7.4), H-17 (2.99, dd , J=10.8, 7.4), H-18 (0.80, s), H-19 (1.06, s), H-21 (7.12, s), H-22 (6.16, s), H-23 (7.32, s), H-28 (0.96, s), H-29 (1.11, s), H-30 (5.30, s), 7-OMe (3.71, s), 12-OAc (1.89, s); NMR Carbon spectrum data δ C (ppm, DMSO-d 6 , 125Hz): 82.6 (CH, 1-C), 4...

Embodiment 2

[0013] Embodiment 2: Pharmacological action test of Turrapubin D

[0014] 1. Materials and Instruments

[0015] HL7702 liver cell line was provided by Shanghai Institute of Biological Sciences, Chinese Academy of Sciences. TurrapubinD is self-made, and the HPLC normalized purity is greater than 98%. High-glucose DMEM medium, high-glucose DMEM / F12=1:1 medium, and fetal bovine serum were all purchased from HycLone. EDTA was purchased from Shanghai Reagent No. 1 Factory. Trypan Blue was purchased from Beijing Chemical Plant. Sugar-free DMEM medium was purchased from Gibco. Trypsin, MTT, and DMSO were purchased from Amresco. LDH release assay kit was purchased from GENMDE. ALT biochemical detection kits, AST biochemical detection kits, and LDH biochemical detection kits were purchased from Beckman Reagent Co., Ltd., USA. Western and IP cell lysates, PMSF, BCA protein concentration determination kits, and MDA detection kits were all purchased from Beyontien Biotechnology Res...

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Abstract

The invention discloses an application of Turrapubin D in preparation of a liver-protecting drug and belongs to the field of medicines. In vitro tests prove that Turrapubin D can enhance tolerance of liver cells to ischemia reperfusion injury in future, and expressions comprise that activity of the liver cells is enhanced and cell death rate is reduced, so that Turrapubin D pre-treatment can alleviate the ischemia reperfusion injury of human liver cells, and Turrapubin D can protect the liver cells and further can be prepared into a liver-protecting drug through research and development.

Description

technical field [0001] The invention relates to a new application of compound TurrapubinD, in particular to the application of TurrapubinD in the preparation of liver-protecting drugs. Background technique [0002] Chun-MaoYuan et al. isolated and purified the compound TurrapubinD for the first time, and published the results in the famous Journal of Natural Products (Bioactive Limonoid and Triterpenoid Constituents of Turraeapubescens, J.Nat.Prod., 2013, 76, 1166-1174). [0003] There is no activity report of this compound yet. Contents of the invention [0004] The object of the present invention is to provide a medical application of TurrapubinD. [0005] The above-mentioned purpose is achieved through the following technical solutions: [0006] The application of TurrapubinD in the preparation of liver-protecting drugs, the chemical structural formula of TurrapubinD is as follows, [0007] detailed description [0008] Below in conjunction with embodiment furth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/343A61P1/16
Inventor 林天样
Owner 林天样
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