A kind of inoculation method of desert algae artificial biological crust and sand fixation
A desert algae and artificial technology, applied in the fields of botanical equipment and methods, horticultural methods, plant cultivation, etc., can solve problems such as weak sand fixation effect, and achieve the effects of reducing water transpiration, making full use of it, and improving compressive strength.
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Embodiment 1
[0041] Use simplified BG11 medium to culture 10,000L Microtheca sheaths in a track-type culture tank with natural light. When the biomass exceeds 0.2g / L, precipitate, remove part of the supernatant and collect to obtain 5,000L of algae pulp; 1,000g of industrial Dissolve high-grade sodium alginate in 100L of water to make a viscous solution with a mass fraction of 1%; mix the algae pulp and sodium alginate evenly for inoculation, and the inoculation amount of desert algae is 2g·DW / m 2 , the dosage of sodium alginate is 1g / m 2 ; Within 15 days after inoculation, when the water content of the sand layer is lower than 20%, use micro-spray watering, spray water once every 3 or 4 hours, each time for 10 or 15 or 20 minutes, and the water flow rate is 30 mL / (m 2 min), stop water spraying after 15 days, and finally realize natural growth; during this process, apply 1 or 2 times of diluted BG11 culture solution according to the situation.
[0042] On the second day after inoculation,...
Embodiment 2
[0044] Use simplified BG11 medium to culture 10,000L Microtheca sheathis and 10,000L Pseudocladon sp. in a track-type culture tank under natural light. When the biomass of both exceeds 0.2g / L, precipitate and remove part of the supernatant before collecting , respectively obtain 5000L algae pulp; two kinds of desert algae are mixed according to the ratio of biomass of 1:1; 2000g industrial grade sodium alginate is dissolved in 100L water to make a viscous solution with a mass fraction of 2%; the algae pulp Mix evenly with sodium alginate for inoculation, the inoculation amount of desert algae is 2g·DW / m 2 , the dosage of sodium alginate is 2g / m 2 ;
[0045] The conventional maintenance procedure for inoculating desert algae is the same as in Example 1.
[0046] On the second day after inoculation, a hard physical-biological mixed crust can be formed on the surface of the sand layer, which can effectively fix the sandy soil. Crust compressive strength is 2.1±0.3 Kg / cm 2 , w...
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