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Tobacco NbFER gene and application thereof in tobacco planting

A tobacco and gene technology, applied in the field of tobacco aroma component synthesis and gene regulation, tobacco NbFER gene

Active Publication Date: 2015-11-11
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no related reports on the application of NbFER in tobacco and its regulation on tobacco plant size and aroma components

Method used

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  • Tobacco NbFER gene and application thereof in tobacco planting
  • Tobacco NbFER gene and application thereof in tobacco planting
  • Tobacco NbFER gene and application thereof in tobacco planting

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Experimental program
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Embodiment Construction

[0020] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and provides a detailed implementation manner and a specific operation process, but the protection scope of the present invention is not limited to the following implementation. example.

[0021] The following experimental methods are conventional methods unless otherwise specified. The reagents used in the following experimental methods were purchased from conventional biochemical reagent companies unless otherwise specified.

[0022] This embodiment includes the following steps:

[0023] 1. Tobacco NbFER gene synthesis and vector construction:

[0024] 1) Primer design: The coding sequences of the PDS and FER genes in N. benthamiana were obtained from NCBI, and the coding sequences of the PDS genes were used as templates, and the sequences located in the middle of the PDS genes were selected as the ...

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Abstract

The invention discloses a tobacco NbFER gene and coded tobacco NbFER functional protein thereof. A tobacco pTRV2-LIC-FERs gene silencing vector is constructed, the vector takes pTRV2-LIC as a skeleton, a segment about 500bp in length is designed in the middle of the NbFER gene and connected into a pTRV2-LIC virus vector, and Ben's tobacco is converted through agrobacterium injection to obtain tobacco with the tobacco NbFER gene silent. Experimental comparison finds that the tobacco NbFER gene can positively adjust the size of tobacco leaves and has an adjusting effect on alcohol, ester and benzene substance, related to aroma, in tobacco, and shows that the NbFER gene has an extremely importance regulating effect in the aspects of tobacco yield and aroma component synthesis.

Description

technical field [0001] The present invention relates to a tobacco aroma component synthesis and regulation gene; in particular to a tobacco NbFER gene, and gene silencing technology in Nicotiana benthamiana NbFER The gene was silenced, and the tobacco with short tobacco leaf plants and high content of aroma components was obtained. Background technique [0002] Tobacco plant size and aroma component content are two very important indicators to measure the quality of tobacco leaves. Aroma substances determine the aroma type, aroma quantity and aroma quality of tobacco. Tobacco aroma substances are divided into tobacco leaf aroma substances and smoke aroma substances, among which tobacco leaf aroma substances include volatile aroma components such as alkanes and polyphenols with relatively simple molecular structures, as well as flavonoids, nicotine, terpenes and other molecular structures Complex aroma precursors. [0003] Since the signing of the Framework Convention on To...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N9/12C12N15/84A01H5/00
Inventor 于峰徐国云汪龙
Owner HUNAN UNIV
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