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Application of extracellular acidic environment in delaying apoptosis of granulocytes

A technology of neutrophils and cells, which is applied in the field of clinical application of new pathways and new targets, and can solve problems such as protracted inflammation and tissue damage

Inactive Publication Date: 2015-10-28
INST OF HEMATOLOGY & BLOOD DISEASES HOSPITAL CHINESE ACADEMY OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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Problems solved by technology

Furthermore, neutrophils have a powerful bactericidal function, and neutrophil infusions have existed 30 years ago and have been proven to be effective in infected patients, but this is a double-edged sword, that is, neutral During the bactericidal process, granulocytes can also cause local tissue damage and some inflammations to persist due to the bystander effect

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  • Application of extracellular acidic environment in delaying apoptosis of granulocytes
  • Application of extracellular acidic environment in delaying apoptosis of granulocytes
  • Application of extracellular acidic environment in delaying apoptosis of granulocytes

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[0041] The present invention will be further described below in conjunction with specific examples, but the protection scope of the present invention will not be limited.

[0042] The invention provides a method for reducing the glutathionylation level of neutrophil actin. The method comprises the following steps: select RPMI 1640 medium to add 10% (volume) fetal bovine serum, and then use hydrochloric acid or carbonic acid Or adjust the pH value with lactic acid: 6.0≤pH value<7.0 (such as pH6.0, pH6.5), filter bacteria with a 0.22 μm filter, and use this medium to culture neutrophils in vitro for 4 hours.

[0043] The present invention also provides a method for in vitro preservation of neutrophils, the method comprising the following steps: select RPMI 1640 medium plus 10% (volume) fetal bovine serum, and then use hydrochloric acid or carbonic acid or lactic acid to adjust the pH value: 6.0≤ The pH value is <7.0 (such as pH6.0, pH6.5), and the bacteria are filtered through a...

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Abstract

The invention relates to application of an extracellular acidic environment in delaying apoptosis of granulocytes. Through inhibiting generation of intracellular active oxygen, the extracellular acidic environment reduces the level of glutathionylation of intracellular globular actin and promotes formation of fibrous actin, thereby activating an Akt signal path and leading to delaying of cell apoptosis. According to the invention, the important role of a microenvironment pH value in immunoloregulation is revealed; a novel path and novel targets revealed in the invention are expected to provide novel ideas and strategies for designing a novel blood transfusion medical storage scheme, a novel neutrophile granulocyte infusion scheme and a novel treatment scheme for diseases and tumors related to inflammations in clinical practice in the future.

Description

Technical field: [0001] The invention belongs to the technical field of drug targets, and mainly relates to a new path for regulating the biological characteristics of human neutrophils and the clinical application of the new targets. Background technique [0002] Neutrophils are derived from bone marrow hematopoietic stem cells, which constitute the forefront of the body's defense against the invasion of foreign pathogens, and play an important role in the body's natural immunity. Neutrophils are the most abundant type of white blood cells in the blood circulation. Different from other white blood cells such as lymphocytes, neutrophils have many unique characteristics. First, neutrophils are short-lived. The half-life of neutrophils in the blood circulation is only 8-20 hours. Senescent neutrophils can undergo constitutive spontaneous apoptosis, which is regulated by various factors inside and outside the cells and has plasticity. Second, neutrophils have chemotaxis and c...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0787C07K14/47
Inventor 许元富曹善楠刘鹏朱海燕公海艳耿广锋王彤
Owner INST OF HEMATOLOGY & BLOOD DISEASES HOSPITAL CHINESE ACADEMY OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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