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Zymomonas mobilis with resistance to acetic acid of high concentration and application thereof

A technology of Zymomonas and movement, applied in the field of microorganisms, can solve the problems of low tolerance to high concentration acetic acid, and achieve the effect of saving detoxification treatment steps

Active Publication Date: 2015-10-28
BIOGAS SCI RES INST MIN OF AGRI
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Problems solved by technology

[0005] In order to overcome the low defect of existing Zymomonas mobilis high concentration acetic acid tolerance, the present invention provides a Zymomonas mobilis bacterial strain that can tolerate up to 8g / L acetic acid

Method used

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  • Zymomonas mobilis with resistance to acetic acid of high concentration and application thereof

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Experimental program
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preparation Embodiment 1

[0026] This preparation example is used to illustrate the preparation of RM medium containing acetic acid, and the process of preparing enzymatic hydrolyzate from raw materials containing cellulose.

[0027] According to 20.0g / L glucose, 10.0g / L yeast powder, 1.0g / L MgCl 2 , 1.0g / L (NH 4 ) 2 SO 4 and 1.0g / L KH 2 PO 4 The formulation configuration of the base RM medium was obtained. Acetic acid was added to the basal RM medium so that the concentrations of acetic acid were 7.5 g / L, 8.0 g / L and 8.5 g / L, respectively.

[0028] Cut the wheat straw into small pieces of about 2 cm, then add water to adjust the water content to 30% by weight; transport it to the heat preservation pressure device, carry out steam explosion at 200 ° C and 1.8 MPa, and collect the steam explosion waste, and the obtained steam The explosive product and water at 80°C were stirred and mixed for 30 minutes according to the weight ratio of 1:3, then added a sulfuric acid solution with a concentration o...

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Abstract

The invention provides zymomonas mobilis with resistance to acetic acid of high concentration , the preservation number of the zymomonas mobilis is CGMCC No. 10945. The zymomonas mobilis provided by the invention can grow rapidly in a culture solution containing more than 8.0g / L of acetic acid, and the alcohol fermentation can be performed rapidly, so that the zymomonas mobilis can perform fermentation on cellulose enzymolysis products directly under higher acetic acid concentration condition to obtain fuel ethanol through preparation.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a high-concentration acetic acid-resistant Zymomonas mobilis and its application. Background technique [0002] Using my country's rich straw resources as raw materials to produce fuel ethanol and other bio-based products is of great significance to promoting the sustainable development of my country's agriculture, expanding agricultural functions and ensuring national energy security. However, in the process of fermenting lignocellulosic biomass resources such as straw to produce ethanol and bio-based chemicals, due to the dense structure between cellulose, hemicellulose and lignin in the raw materials, it is not conducive to the subsequent enzymatic saccharification of cellulose . Therefore, before enzymatic hydrolysis, lignocellulosic biomass generally needs to undergo physical and chemical pretreatment methods to improve the saccharification efficiency of cellulase on cellu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/10C12R1/01
CPCC12P7/10C12N1/205C12R2001/01Y02E50/10
Inventor 何明雄税宗霞祝其丽秦晗张敏吴波谭芙蓉代立春
Owner BIOGAS SCI RES INST MIN OF AGRI
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