Composition capable of blocking generation of nitrosamine and killing helicobacter pylori
A technology of Helicobacter pylori and composition, which is applied in the formulation field of health care products, and can solve the problems of carcinogenicity and cancer occurrence in animals or humans
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Embodiment 1
[0025] Mix 60g of xylitol, 0.5g of orange essence, 0.1g of orange pigment, 10g of silicon dioxide, 10g of magnesium stearate and 19.6g of light calcium carbonate powder to form a 100g matrix.
[0026] Add 600 g of L-ascorbyl palmitate, 300 g of L-ascorbic acid and 50 g of cysteine into the 50 g of the base, and stir while adding, so that they are uniformly mixed with the base. That is, 1000 g of this composition was obtained.
Embodiment 2
[0028] Get 6 stoppered test tubes of 50ml and number them, add 10.0ml concentration respectively to be the sodium nitrite solution of 200 μ g / ml, the citric acid-disodium hydrogen phosphate buffer solution of 10.0ml pH=3.0 and this composition 10.0mg (control group Add 10.0 mg of calcium carbonate powder to the test tube), then add deionized water to the mark, mix well, and place it in a constant temperature water bath at 37°C for 30 minutes.
[0029] The content of residual nitrite in the treated samples was determined according to the method of "Determination of Nitrite and Nitrate in Food GB 5009.33-2010 Second Method Spectrophotometry".
[0030] Experimental results show that the composition has a very good nitrite removal effect, and residual nitrite is no longer detected in samples treated with the composition.
Embodiment 3
[0032] Take six 50ml stoppered test tubes and number them, add 10.0ml sodium nitrite solution with a concentration of 1mmol / l, 10.0ml dimethylamine with a concentration of 1mmol / l, and 10.0ml citric acid-hydrogen phosphate with a pH=3.0 Sodium buffer solution and 10.0 mg of this composition (10.0 mg of calcium carbonate powder was added to the test tube of the control group), then add deionized water to the mark, mix thoroughly, and place in a constant temperature water bath at 37°C for 60 minutes.
[0033] Use a pipette gun to draw 10.0ml of the above solution into a 50ml beaker, add 0.5% Na 2 CO 3 Solution 5ml terminates above-mentioned reaction, then according to " the determination of N-nitrosamines in food GB 5009.26-2003 second method gas chromatography mass spectrometer method " method is measured the content of the N-nitrosodimethylamine in the processed sample content.
[0034] Experimental results show that the composition has a very good blocking effect on N-nitro...
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