Rapid Propagation Method of White Uncaria
A kind of white Uncaria, fast technology, applied in the field of rapid reproduction of white Uncaria, to achieve the effect of improving tissue cell division, promoting formation, and accelerating growth rate
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Embodiment 1
[0035] A kind of rapid propagation method of white hook vine, comprising the following steps:
[0036] Step 1. Make sterile explants of Uncaria uncariae, select the tender stem section with joints close to the terminal buds of Uncaria uncariae, remove excess leaves, gently scrub the surface of the explants with a fine brush under running water, and then cut them into 2cm Long shoots with axillary buds were used as sterile explants of Uncaria uncariae. Disinfect the explants of Uncaria uncariae, the specific disinfection process is: soak the explants with a mixed solution of allicin and ethanol with a mass ratio of 1:100 for 5 minutes, and then rinse the belt joints with sterile water. Tender stem section three times, then soak the lower 1 / 3 of the explant and the lower part in bamboo vinegar with a mass fraction of 1% for 25min, then continue to rinse the explant with sterilized tea water 20s.
[0037]Step 2, transfer the sterile explants into the first culture medium for li...
Embodiment 2
[0048] A kind of rapid propagation method of white hook vine, comprising the following steps:
[0049] Step 1. To make sterile explants of Uncaria uncariae, select the tender stem section with joints close to the terminal buds of Uncaria uncariae, remove the excess leaves, gently scrub the surface of the explants with a fine brush under running water, and then cut them into 4cm Long shoots with axillary buds were used as sterile explants of Uncaria uncariae. Disinfect the explants of Uncaria uncariae, the specific disinfection process is: soak the explants with a mixed solution of allicin and ethanol with a mass ratio of 1:100 for 5 minutes, and then rinse the belt joints with sterile water. Tender stem section three times, then soak the lower 1 / 3 of the explant and the lower part in bamboo vinegar with a mass fraction of 1% for 30min, then continue to rinse the explant with sterilized tea water 30s.
[0050] Step 2. Transfer the sterile explants into the first culture mediu...
Embodiment 3
[0061] A kind of rapid propagation method of white hook vine, comprising the following steps:
[0062] Step 1. To make sterile explants of Uncaria uncariae, select the tender stem section with joints close to the terminal buds of Uncaria uncariae, remove excess leaves, gently scrub the surface of the explants with a fine brush under running water, and then cut them into 3cm Long shoots with axillary buds were used as sterile explants of Uncaria uncariae. Disinfect the explants of Uncaria uncariae, the specific disinfection process is: soak the explants with a mixed solution of allicin and ethanol with a mass ratio of 1:100 for 5 minutes, and then rinse the belt joints with sterile water. Tender stem section three times, then soak the lower 1 / 3 of the explant and the lower part in bamboo vinegar with a mass fraction of 1% for 26 minutes, then continue to rinse the explant with sterilized tea water 26s.
[0063] Step 2, transfer the sterile explants into the first culture medi...
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