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Purification method of maytenfolic acid

A purification method, the technology of folic acid, applied in the field of natural medicinal chemistry, can solve the problems of low product purity, complicated operation, difficult industrial production, etc., and achieve the effect of high product purity and simple and easy operation

Inactive Publication Date: 2015-09-30
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complicated operation, small amount of preparation and low purity of the obtained product, it is difficult to realize industrial production

Method used

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  • Purification method of maytenfolic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take the dried euonymus and grind it, pass through a 20-mesh sieve, and collect the coarse powder for later use; dissolve the cellulase in distilled water according to the ratio of 0.1g cellulase / L water, stir evenly, add hydrochloric acid solution dropwise, and adjust the pH value 4. Add euonymus coarse powder into the above-mentioned cellulase aqueous solution at a mass volume ratio of 1:1, stir evenly, heat in a water bath to 45°C, perform constant temperature enzymatic hydrolysis for 1 hour, then raise the temperature to 60°C, heat for 20 minutes, and inactivate the enzyme , to obtain the enzymatic hydrolysis mixture; add a solid-liquid ratio of 1:3, 30% ethanol aqueous solution to the above enzymolysis mixture, conduct ultrasonic extraction at a frequency of 20KHz, and a temperature of 50°C, filter, and ultrasonically extract the filter residue once, and combine the filtrates Concentrate under reduced pressure until there is no alcohol smell, collect the concentrate...

Embodiment 2

[0023] Take the dried euonymus raw material and grind it through a 40-mesh sieve to collect the coarse powder for later use; dissolve the cellulase in distilled water according to the ratio of 0.3g cellulase / L water, stir evenly, add hydrochloric acid solution dropwise, and adjust the pH value to 4. Add euonymus coarse powder into the aqueous solution of cellulase at a mass volume ratio of 1:3, stir evenly, heat in a water bath to 45°C, perform constant temperature enzymolysis for 1.5h, then raise the temperature to 70°C, heat for 30min, and inactivate the enzyme , to obtain the enzymolysis mixture; add a solid-liquid ratio of 1:4, 30% ethanol aqueous solution to the above enzymolysis mixture, perform ultrasonic extraction at a frequency of 40KHz and a temperature of 60°C, filter, and ultrasonically extract the filter residue twice, and combine The filtrate was concentrated under reduced pressure until there was no alcohol smell, and the concentrated solution was collected for ...

Embodiment 3

[0025] Take the dried euonymus raw material and pulverize it, pass through a 60-mesh sieve, and collect the coarse powder for later use; dissolve the cellulase in distilled water according to the ratio of 0.5g cellulase / L water, stir evenly, add hydrochloric acid solution dropwise, and adjust the pH value 5. Add euonymus coarse powder into the aqueous solution of cellulase at a mass volume ratio of 1:5, stir evenly, heat in a water bath to 50°C, perform constant temperature enzymolysis for 2 hours, then raise the temperature to 80°C, heat for 40 minutes, and inactivate the enzyme. The enzymolysis mixture is obtained; add a solid-liquid ratio of 1:5, 30% ethanol aqueous solution to the enzymolysis mixture, perform ultrasonic extraction at a frequency of 40KHz and a temperature of 70°C, filter, and ultrasonically extract the filter residue for 3 times, and combine the filtrates to reduce Concentrate under pressure until there is no alcohol smell, collect the concentrated solution...

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Abstract

The invention discloses a purification method of maytenfolic acid. The method comprises the following steps: pulverizing a dry Euonymus raw material, screening, adding an enzymolysis water solution, carrying out enzymolysis under acidic conditions for 1-3 hours, after the enzymolysis finishes, adding a extraction solvent, carrying out ultrasonic extraction, filtering, extracting the filter residue 1-3 times, merging the extracting solutions, concentrating under reduced pressure until no alcohol smell, eluting the concentrated solution through a macroporous adsorbent resin column, collecting the eluate, purifying high-speed counter-current chromatography, collecting the target component according to the spectrum, recovering the reagent, and carrying out freeze-drying to obtain the maytenfolic acid. The method for maytenfolic acid is simple in technical operation and low in cost, and can easily implement industrial production.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, in particular to a method for purifying maytans folic acid. Background technique [0002] Maytans folic acid is a triterpenoid compound with anti-inflammatory and anti-cancer activities, CAS number 84108-17-8, molecular formula C 30 h 48 o 4 , the molecular weight is 472.7, and the molecular structure formula is as follows: [0003] [0004] Euonymus alatus (Thunb.) Sieb. is a Euonymus alatus (Thunb.) Sieb., a plant of the family Euonymus. It is used as medicine with roots, winged branches and leaves, and has anti-inflammatory, anti-tumor and hypoglycemic activities. [0005] Through literature search, the existing method for preparing maytans folic acid is to use organic reagent extraction and silica gel separation methods. Due to complicated operation, small amount of preparation and low purity of the obtained product, it is difficult to realize industrialized production. Con...

Claims

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Application Information

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IPC IPC(8): C07J63/00
CPCC07J63/00C07H1/08C07H15/256
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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