A Seedling Transition Method for Improving the Transplanting Survival Rate of Tobacco K326 Tissue Cultured Seedlings

Abstract

The invention discloses a transition method for improving the transplanting survival rate of tobacco K326 tissue culture seedlings. The root culture medium was removed from the seedlings, the whole plant was immersed in carbendazim solution for disinfection, then rinsed with water, and then the tissue culture seedlings were transplanted into the sterilized pot soil. After growing in the sterilized pot soil, it was transferred to an outdoor shaded place to continue growing for several weeks. Finally, it was transferred to a normal open air environment, and watering and fertilizing management were carried out as usual, that is, the transition of tobacco K326 tissue culture seedlings was completed. The invention can greatly improve the survival rate of tobacco transgenic tissue culture seedlings after outdoor transplanting to 96.7-100%, reduce workload, save time, energy consumption and cost. The invention can shorten the time of seedling hardening, and can complete the process from seedling hardening, disinfection to transplanting and potting in one week.

Description

technical field [0001] The invention relates to a seedling hardening transition method for improving the transplanting survival rate of tobacco K326 tissue cultured seedlings, and belongs to the technical field of plant breeding and cultivation. Background technique [0002] tobacco( Nicotiana tabacum ) is an important annual or perennial economic crop of Nicotiana in the family Solanaceae. Tobacco has become an important model plant in the field of plant genetic engineering due to its easy tissue culture and high transformation efficiency. Tobacco K326 was introduced from the United States to Yunnan, my country in 1985. Its advantage is that its aroma quality and aroma are superior to other tobacco varieties. It is currently the variety with the largest planting area in my country, and it is also widely used as an important model in the verification of plant gene functions. one of the plants. [0003] Tobacco, as a model plant, is widely used in experiments for the functio...

AI Technical Summary

Problems solved by technology

[0004] At present, in the general outdoor hardening transition process of tobacco K326 tissue culture seedlings, it is often only a simple and extensive indoor hardening process, and only a certain concentration of fungicides such as carbendazim are used for a certain period of time. In the stage of indoor seedling hardening, the tissue cultured seedlings did not go through a continuous and gradual transitional hardening process, or fungicides such as carbendazim were not effective in killing pathogens in the external environment such as the cultivation soil, or due to poor operation during the transplanting process. Planning and late management of tobacco tissue cultured seedlings just after they are transplanted into pots, so in the past, tobacco transgenic tissue cultured seedlings often died after being transferred from the tissue culture room, during the transition process of seedling hardening and after transplanting The survival rate is too high, the survival rate does not exceed 70%, and in severe cases, the entire batch of seedlings will die after transplanting.

Images