A regional source apportionment method for microorganisms carried by air particles

Abstract

The invention discloses a regional source analytic method for microorganisms carried by air particulate matters. The method comprises a first step of collection of air particulate matter samples and regional typical surface dust samples; a second step of extraction and high-throughput sequencing of microorganism DNAs; a third step of sample community structure analysis; a fourth step of principal coordinate analysis and community similarity analysis; a fifth step of whole potential regional source analysis of microorganisms carried by air particulate matters; and a sixth step of source analysis of pathogenic bacteria and sensitization bacteria. The method is used for the survey of the potential regional sources of the microorganisms carried on the surfaces of air particulate matters, can analyze the relationship between the communities of the microorganisms carried on the surfaces of TSP, PM10 and PM2.5 and other suspended particulate matters of multiple particle sizes and the microbial communities of the regional surrounding surface samples, thereby analyzing the haze source in the view of microbial communities, and providing theoretical guidance for prevention of the haze. Regional source analysis of the pathogenic microorganisms and sensitization bacteria in air particulate matters is of great significance on health of human being and hygiene and disease control.

Description

technical field [0001] The invention relates to an area source analysis method for microorganisms carried by air particles. Background technique [0002] Due to human activities and various meteorological conditions, atmospheric aerosol fine particle pollution is becoming more and more serious, and haze weather is becoming more and more frequent. The frequent occurrence of haze weather has attracted the attention of many researchers. At present, the research mainly focuses on the analysis of physical and chemical factors of haze air particles, the contribution sources of atmospheric aerosol fine particles in cities, and the construction of air pollution models. There are not many studies on the analysis of differences in the composition of microbial communities in the air and in the environment, and the analysis of the source of airborne microorganisms. Existing studies have shown that the microorganisms carried by particulate matter in the air, especially pathogens and all...

AI Technical Summary

Problems solved by technology

The frequent occurrence of haze weather has attracted the attention of many researchers. At present, the research mainly focuses on the analysis of physical and chemical factors of haze air particles, the contribution sources of atmospheric aerosol fine particles in cities, and the construction of air pollution models. There are not many studies on the analysis of the differences in the composition of microbial communities in the air and the environment and the analysis of the source of airborne microorganisms

Images