Method for continuously preparing phellinus igniarius mycelium polysaccharide

Abstract

The invention relates to a method for continuously preparing phellinus igniarius mycelium polysaccharide, belonging to the technical field of bioengineering. The method comprises the following steps: extracting degreased phellinus igniarius mycelium in 95-DEG C hot water under reflux for 8 hours, centrifuging, concentrating, precipitating with ethanol, removing proteins, dialyzing, and carrying out freeze-drying to obtain water-extraction phellinus igniarius mycelium polysaccharide; dissolving the water-extraction residue in a 1% ammonium oxalate solution, extracting at 95 DEG C under reflux for 8 hours, centrifuging, concentrating, precipitating with ethanol, removing proteins, dialyzing, and carrying out freeze-drying to obtain acid-extraction phellinus igniarius mycelium polysaccharide; and dissolving the acid-extraction phellinus igniarius mycelium polysaccharide in 1.25 mol / L sodium hydroxide and 0.05% sodium borohydride solution, extracting at normal temperature for 3 hours, centrifuging, concentrating, adding acetic acid, precipitating with ethanol, removing proteins, dialyzing, and carrying out freeze-drying to obtain alkali-extraction phellinus igniarius mycelium polysaccharide. The phellinus igniarius mycelium polysaccharide continuously prepared by the method has obvious free-radical removal capacity and antioxidation activity. The preparation method is simple and easy to implement, is low in cost, can implement large-scale production, and can be used for other fungus mycelia, such as ganoderma lucidum, aweto, Grifola frondosa and the like.

Description

technical field [0001] The invention relates to a method for continuously preparing Phellinus mycelium polysaccharide, which belongs to the technical field of bioengineering. Background technique [0002] Phellinus ( Phellinus linteus ) of the Basidiomycotina ( Basidiomycotina ), Polyporaceae, Xylinophores ( Phellinus ), is a rare medicinal fungus known as "forest gold" and widely used in East Asia, especially Korea, China and Japan. Phellinus polysaccharide is the main bioactive component, which has various physiological activities and medicinal value such as immune regulation, anti-tumor, anti-inflammation, anti-allergic, anti-angiogenesis and anti-oxidation. Due to the influence of its own biological characteristics and the external environment, wild Phellinus fungus fruiting bodies are scarce, and the artificial cultivation period is long, which cannot meet people's extensive demand for rare Phellinus japonica resources. Industrialization development provides the p...

AI Technical Summary

Problems solved by technology

Due to the influence of its own biological characteristics and the external environment, the wild Phellinus sporocarpa is scarce, and the artificial cultivation period is long, which cannot meet people's extensive demand for rare Phellinus resources. Industrial development provides the possibility, and the fermentation cycle is short, not limited by seasons and environments, and the efficacy of mycelium polysaccharides obtained by fermentation is equivalent to that of fruiting body polysaccharides

However, at present, domestic and foreign studies on Phellinus mycelium polysaccharides are mainly based on water extracts or water-extracted polysaccharides, and the residues after water extraction are generally discarded, resulting in a great waste of biomass energy and limiting the production of Phellinus active polysaccharides. In-depth development and utilization

[0003] After searching, there is no report about the preparation of Phellinus mycelium polysaccharide in the residue after water extraction