Capillary column system capable of increasing open tubular column sample loading quantity by variable-diameter packing column

Abstract

The invention relates to a capillary column system capable of increasing the open tubular column sample loading quantity by a variable-diameter packing column. The capillary column system mainly comprises the variable-diameter packing column, a connecting piece and an open tubular column. The capillary column system is characterized in that the variable-diameter packing column is fixed on an internal fingertight fitting of the connecting piece, the open tubular column passes through an external fingertight fitting to be in seamless connection with the tip of the packing column, a dead volume is zero, the internal diameter of the inlet end of the variable-diameter packing column is 320-530 micrometers, the internal diameter of the outlet end of the variable-diameter packing column is 10-75 micrometers, and the length of the variable-diameter packing column is 1-5cm. The capillary column system capable of increasing the open tubular column sample loading quantity by the variable-diameter packing column has the advantages that the variable-diameter packing column is used as a sample loading column, and the advantage of large sample loading quantity of the packing column can be used fully for increasing the sample loading quantity of the open tubular capillary column system; a short variable-diameter packing column can be adopted, so that detecting sensitivity and repeatability are improved while the sample loading quantity is increased; zero connecting dead volume can be guaranteed by the aid of a special structure of the connecting piece and diameter varying of the packing column.

Description

【Technical Field】 [0001] The invention relates to the technical field of capillary column systems, in particular to a capillary column system that utilizes a variable-diameter packed column to increase the sample amount of an open-tube column. 【Background technique】 [0002] The capillary open-tube column is a chromatographic separation column with a special hollow structure. Its stationary phase is fixed on the inner surface of the capillary through chemical bonding or physical coating. The unique structure makes it have the advantages of high column efficiency, small column back pressure, and suitable for rapid separation, and it avoids the complexity of the overall column preparation process and the lack of control of repeatability [Document 1.Won Jo Cheong et al. Journal of Chromatography A, 2013, 1308: 1-24], so the preparation and research of open-tube columns have always attracted people's attention. However, the open-tube capillary column has always had the defect of ins...

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Problems solved by technology

Although the two-dimensional capillary system method can increase the amount of sample to a large extent, the capillary used is of equal diameter, and there is a problem that the sample loading column is too long, which affects the repeatability and separation efficiency of the column system, and the spliced ​​two-dimensional The connection of the three-dimensional capillary system has a large dead volume, which affects the detection sensitivity and is not conducive to popularization

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