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Quick detection method for a variety of pathogen molecules of strawberry root rot diseases and application of quick detection method

A detection method and pathogenic bacteria technology, which is applied in the field of rapid molecular detection of various pathogenic bacteria of strawberry root rot, can solve problems such as effective methods, and achieve the effects of large throughput, avoiding transmission and occurrence, and less time-consuming

Inactive Publication Date: 2015-08-19
QINGDAO AGRI UNIV
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Problems solved by technology

At present, there is no effective method to control strawberry root rot

Method used

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  • Quick detection method for a variety of pathogen molecules of strawberry root rot diseases and application of quick detection method
  • Quick detection method for a variety of pathogen molecules of strawberry root rot diseases and application of quick detection method
  • Quick detection method for a variety of pathogen molecules of strawberry root rot diseases and application of quick detection method

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Embodiment Construction

[0029] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0030] 1. Isolation of pathogenic bacteria

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Abstract

The invention discloses a quick detection method for a variety of pathogen molecules of strawberry root rot diseases and application of the quick detection method. The method is characterized in that DNA (Deoxyribose Nucleic Acid) of pathogen is subjected to PCR (Polymerase Chain Reaction) reaction by using a general specific primer of pathogenic fungus; when a single strip of about 500bp is represented by a PCR product, the PCR product is subjected to sequencedsequencing; the sequencing result is compared in an NCBI (National Center For Biotechnology Information) database, so that different pathogens which are contained in a disease sample tissue and cause the strawberry root rot diseases can be defined. The method has the advantages of simplicity in operation, short time and large flux; the method can be used for qualitatively detecting root part disease samples of seedlings in a strawberry nursery, so that the spread and the incidence of the strawberry root rot diseases can be effectively avoided, and the construction of strawberry disease-free seedling production bases and safe production of the strawberry disease-free seedling in China are guaranteed.

Description

technical field [0001] The invention belongs to the technical field of fruit tree disease prevention and plant quarantine, and relates to a molecular rapid detection method and application of various pathogenic bacteria of strawberry root rot. Background technique [0002] Strawberry root rot is a general term for a large class of diseases caused by soil-borne pathogenic fungi. More than 20 species of root rot pathogens have been reported in major strawberry producing areas around the world, and it is a soil-borne disease that is difficult to control. Common pathogenic bacteria are: the main causes of strawberry black root rot are Fusarium spp., F.oxysporum, Rhizoctonia solani, Pythium sp., Pestalotiopsis sp.; Phytophthorafragariae C.J.Hickman is the pathogen that causes root rot of strawberry red stem. The typical symptom of strawberry root rot is that when the diseased root is cut or the outer skin of the root is peeled off, dark red diseased tissue can be seen, which is...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12R1/645
CPCC12Q1/686C12Q2565/125
Inventor 黄金光孙晓梅
Owner QINGDAO AGRI UNIV
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