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Method for preparing gluten antihypertensive peptide based on sequential ultrasonic enhanced enzymolysis

A blood pressure-lowering peptide, ultrasonic enhancement technology, applied in the field of bioengineering

Active Publication Date: 2015-08-19
江苏江大五棵松生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor solubility and poor dispersion of the hydrophobic amino acid content of gluten protein (mainly containing high content of Pro, Gln, Leu, and Ala), the gluten protein cannot be fully combined with the enzyme, and the enzymatic hydrolysis efficiency is low.

Method used

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  • Method for preparing gluten antihypertensive peptide based on sequential ultrasonic enhanced enzymolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1. Prepare 6 L of 2% gluten protein suspension, under the condition of mechanical stirring, the frequency is 20 kHz, 28 kHz, 35 kHz, the three-frequency ultrasonic time is 20s, 20s, 20s; the ultrasonic power is 16.67 W / L, Divergent ultrasonic treatment at an initial temperature of 30°C for 30 min; adding alkaline protease at a rate of 3800 U / g for enzymolysis at a temperature of 50°C and a pH of 9.0 for 30 min; after enzymolysis, the The enzymolysis solution was boiled for 10 minutes to inactivate the enzyme, centrifuged at 12000 r / min for 10 minutes, and the supernatant was collected to obtain the extract of the protein blood pressure lowering peptide. The gluten protein hypotensive peptide extract was diluted 2 times to determine the ACE inhibitory activity.

[0024]After measurement, the ACE inhibition rate of the dilute enzymatic hydrolyzate with ultrasonic treatment was 73.80%, and compared with the control (1), the blood pressure lowering activity was i...

Embodiment 2

[0025] Example 2. Prepare 6 L of 3% gluten protein suspension, under the condition of mechanical stirring, the frequency is 20kHz, 28kHz, 35kHz, the three-frequency ultrasonic time is 20s, 20s, 20s; the ultrasonic power is 16.67 W / L, and the initial temperature is Divergent ultrasonic treatment at 20°C for 30 min; add alkaline protease at a rate of 3800 U / g for enzymolysis, the temperature of enzymolysis is 50°C, pH is 9.0, and enzymolysis is 30 minutes; after enzymolysis, the enzymolysis The solution was boiled for 10 min to inactivate the enzyme, centrifuged at 12000 r / min for 10 min, and the supernatant was collected to obtain the extract of the protein blood pressure lowering peptide. The gluten protein hypotensive peptide extract was diluted 3 times to determine the ACE inhibitory activity.

[0026] After measurement, the ACE inhibition rate of the dilute solution of the enzymatic hydrolyzate treated with ultrasonic treatment was 77.73%, and the blood pressure lowering...

Embodiment 3

[0027] Example 3. Prepare 6 L of 3% gluten protein suspension, under the condition of mechanical stirring, the frequency is 20 kHz, 28 kHz, 35 kHz, the single-frequency ultrasonic time is 20s, 20s, 20s; the ultrasonic power is 16.67 W / L, The initial temperature was 20°C and diffuse ultrasonic treatment for 50 minutes; alkaline protease was added at a ratio of 3800 U / g for enzymolysis, the temperature of enzymolysis was 50°C, pH was 9.0, and the enzymolysis was 30 minutes; after the end of enzymolysis, The enzymolysis solution was boiled for 10 minutes to inactivate the enzyme, centrifuged at 12000 r / min for 10 minutes, and the supernatant was collected to obtain the extract of the protein blood pressure lowering peptide. The gluten protein hypotensive peptide extract was diluted 3 times to determine the ACE inhibitory activity.

[0028] After measurement, the ACE inhibition rate of the dilute solution of the enzymatic hydrolyzate treated with ultrasonic treatment was 76.76%...

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Abstract

The invention provides a method for preparing gluten antihypertensive peptide based on sequential ultrasonic enzymolysis, which belongs to the technical field of deep processing of agricultural and sideline products. The method comprises the following steps: carrying out ultrasonic treatment on gluten suspension, wherein frequencies of sequential tri-frequency ultrasonic waves are 20 KHz, 28 KHz and 35 KHz, ultrasonic power is 16.67 W / L and pretreatment time is 20 to 50 min; after pretreatment, adding protease for enzymolysis, wherein during enzymolysis, a pH value is maintained to be 9.0, temperature is maintained to be 50 DEG C, and enzymolysis time is 30 min; and carrying out enzyme deactivation at 100 DEG C for 10 min, then carrying out centrifugation at 12000 r / min for 10 min and collecting supernatant. According to the invention, the gluten suspension is pretreated by using tri-frequency sequential ultrasonic waves, so enzymolysis efficiency is improved; and compared with other ultrasonic pretreatment manners, the ACE inhibition rate of enzymatic hydrolysate is increased by 13 to 20% and energy consumption is reduced by 3 to 53.75 times.

Description

Technical field: [0001] The invention relates to the field of bioengineering, in particular to a technique for pretreating gluten protein raw materials with sequential triple-frequency ultrasonic waves, and then using an enzymatic hydrolysis method to prepare gluten protein blood pressure-lowering peptides. technical background: [0002] Wheat gluten is a by-product of wheat starch processing. Its protein content is as high as 75-80%, and its amino acid composition is balanced and complete. It is a plant-based high-protein polymer with rich nutrition and low price. The annual output of wheat gluten in my country is about 600,000 tons. At present, it is mainly used in the food and feed industry as flour quality improver, feed binder, and nutritional additive. The latest research has found that gluten protein is a very potential plant protein source for the preparation of bioactive peptides, and its enzymatic hydrolysis product can reduce blood pressure by significantly inhi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 张艳艳马海乐任晓锋李云亮王蓓曲文娟王凯
Owner 江苏江大五棵松生物科技有限公司
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