Novel bacterial RNA (ribonucleic acid) extraction reagent and preparing method thereof

Abstract

The invention relates to a novel bacterial RNA (ribonucleic acid) extraction reagent and a preparing method thereof. The reagent is composed of bacterial cell wall breaking reagent, binding liquid and cleaning solution. The bacterial cell wall breaking reagent is composed of extract A and extract B. The extract A is a mixture of Tris, EDTA (ethylene diamine tetraacetic acid), SDS (sodium dodecyl sulfate), Tween 80, Triton X-100, glycine, sarcosyl, Beta-mercaptoethanol and water; the extract B is a mixture of water phenol, ethanol and pyrrolidone. The binding liquid is guanidinium isothiocyanate solution. The cleaning solution is ethanol water. The novel bacterial RNA extraction reagent has the advantages that during extraction of total RNA of the gram-positive bacterium, the content of total RNA in the finally acquired gene group DNA and total RNA is evidently increased, extraction efficiency of the total RNA is increased, and wide application is benefited.

Description

technical field [0001] The invention relates to an RNA extraction reagent and a preparation method thereof, in particular to a novel bacterial RNA extraction reagent and a preparation method thereof. Background technique [0002] RNA is a messenger or template that exists in biological cells to transfer genetic information from DNA to functional proteins. In recent years, with the progress of bacterial genome engineering, we have ushered in the post-genome era of bacteria. It has become a hot research topic to explain the function of genes and the regulation mechanism of gene expression through the study of gene expression profiling. RNA extraction technology is not only an important part of molecular biology technology, but also an important basis for functional genomics research technology. With the advent of the post-genome era of bacteria, seeking an effective reagent and method for extracting bacterial RNA has become an urgent problem to be solved. [0003] CN10279672...

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Problems solved by technology

[0004] Although the above-mentioned prior art discloses some bacterial RNA extraction reagents, which can meet certain needs, there are still certain defects in these bacterial RNA extraction reagents: in the genomic DNA and total RNA extracted by the above method, the genomic DNA and total RNA The amount of total RNA is about half, and the content of total RNA is low, indicating that the efficiency of the above extraction reagents in the extraction of total RNA from Gram-positive bacteria is low, which affects the use