Printing method for three-dimensional biological structure with built-in nutrition channel

Abstract

The invention discloses a printing method for a three-dimensional biological structure with a built-in nutrition channel. The method includes: forming the hollow gelatinous fibers which are not completely reacted and contain cells by controlling the technological parameters during printing, and building the three-dimensional biological structure with the built-in nutrition channel by utilization of the principle that the gel which is not completely reacted can be fused. The printing method utilizes the three-dimensional printing device which is provided with a coaxial sprayer and a z-shaped plate, produces the hollow gelatinous fibers which are not completely reacted through the coaxial sprayer as the basic unit of the three-dimensional structure, drives the X-axis and the Y-axis to realize the printing of each layer of structures, realizes layers of printing through the lowering of the z-shaped plate and enables the lower-layer of the gel structure to be completely reacted to be used as the support for the upper layer. The printing method for the three-dimensional biological structure with the built-in nutrition channel can realize simultaneous printing of the supporting structure and the internal flow channel, and is beneficial for the cell survival.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a printing method of a three-dimensional biological structure with built-in nutrition channels. Background technique [0002] Currently, the number of patients waiting for organ transplants worldwide is staggering every year. More than 6,500 people in the U.S. die each year due to delayed access to donated organs. In addition, even if donated organs are obtained, patients will experience varying degrees of rejection after receiving transplantation techniques. At present, there are two main methods of making organs. One is to use the idea of ​​tissue engineering to print scaffolds and then culture cells in the scaffolds, and the other is to use cells to directly print to form organs. [0003] At present, the idea of ​​making organs based on tissue engineering technology is to make porous scaffolds first, then culture cells in the scaffolds, and finally tra...

AI Technical Summary

Problems solved by technology

However, the tissue engineering method is difficult to solve the problem of cell density, and the cells grow on the surface of the scaffold, while the real cell growth environment is three-dimensional, and more importantly, the cells on the surface can get more nutrients, and the cells in the inner layer are malnourished

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