Method for extracting and separating allelopathic autotoxicity substances from panax notoginseng rhizosphere soil

A technology for rhizosphere soil and autotoxic substances, applied in the field of extracting allelopathic autotoxic substances, can solve problems such as the influence of microbial community structure, plant growth inhibition, death, etc., and achieve high extraction efficiency, reduce interference and loss, and low toxicity. Effect

Inactive Publication Date: 2015-07-29
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

Although these compounds have small molecular weight and simple structure, when released into the soil environment, they will not only change the physical and chemical properties of t...

Method used

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  • Method for extracting and separating allelopathic autotoxicity substances from panax notoginseng rhizosphere soil
  • Method for extracting and separating allelopathic autotoxicity substances from panax notoginseng rhizosphere soil
  • Method for extracting and separating allelopathic autotoxicity substances from panax notoginseng rhizosphere soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 methanol extraction

[0027] (1) Soil sample collection: Soil collection was carried out in the land planted with three-year-old Sanqi through the cross five-point method, and soil samples of 0-30 cm in the rhizosphere of the Panax notoginseng plant were taken.

Embodiment 2

[0030] Embodiment 2 (contrast 1-80% methanol extraction)

[0031] Preparation method of 80% methanol extract: prepare a methanol solution with a volume fraction of 80%, weigh 20 g of the sample 2 described in Example 1 with an electronic balance, place it in 200 ml of 80% methanol by volume fraction, and process it ultrasonically for 30 minutes. Filtrate with medium-speed filter paper and collect the filtrate. The collected filtrate is the extracted allelopathic autotoxic substance extract. The filtrate is concentrated with a rotary evaporator until the filtrate is 5ml, stored at 4°C, and the concentration temperature is 50°C. It is to be tested.

Embodiment 3

[0032] Embodiment 3 (contrast 2-70% methanol extraction)

[0033] Preparation method of 70% methanol extract: configure a methanol solution with a volume fraction of 70%, weigh 20 g of the sample 3 described in Example 1 with an electronic balance, place it in 200 ml of 70% methanol by volume fraction, and process it ultrasonically for 30 minutes. Filtrate with medium-speed filter paper and collect the filtrate. The collected filtrate is the extracted allelopathic autotoxic substance extract. The filtrate is concentrated with a rotary evaporator until the filtrate is 5ml, stored at 4°C, and the concentration temperature is 50°C. It is to be tested.

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Abstract

The invention discloses a method for extracting and separating allelopathic autotoxicity substances from panax notoginseng rhizosphere soil. The method comprises steps as follows: soil samples are collected and treated, 20 g of the treated soil samples are weighed, extracted with methanol and subjected to ultrasonic processing, and the allelopathic autotoxicity substances are separated with an high performance liquid chromatography method; the chromatographic conditions are as follows: the chromatographic instrument adopts Agilent 1260, the chromatographic column adopts specifications of AgilentZorbax SB-C18, 250mm*4.6mm and 5 mu m, the mobile phase A is an H3PO4 aqueous solution with mass fraction of 0.1%, the mobile phase B is methanol, the flow speed is 1.0ml/min, the column temperature is 25 DEG C, the detecting wavelength is 203 nm, and gradient elution is performed. An experiment proves that the maximum varieties of the allelopathic autotoxicity substances are obtained through extraction and separation with the method; the method is simple and convenient to implement, and disturbance and loss caused by an extraction solvent to target compounds in samples as a result of a complicated extraction technology in the sample extraction process are reduced.

Description

technical field [0001] The invention belongs to the method in the technical field of planting medicinal plants, and in particular relates to a method for extracting allelopathic autotoxic substances from the rhizosphere soil of Panax notoginseng. technical background [0002] Radix Notoginseng [Panax notoginseng (Burk) F.H.Chen] is a plant of the genus Ginseng in the Araliaceae family. Its roots and rhizomes are used as medicine. It has the effects of promoting blood circulation, removing blood stasis, reducing swelling and relieving pain. At present, Panax notoginseng has been widely used. Statistics show that in China alone, more than 1,300 enterprises use Panax notoginseng as raw material, obtain 3,207 production approvals, and produce more than 400 products, including more than 300 types of Chinese patent medicines, covering almost all All traditional Chinese medicine pharmaceutical companies. [0003] Panax notoginseng originated in the Tertiary paleotropical mountaino...

Claims

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Application Information

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IPC IPC(8): G01N30/06
Inventor 陈军文孙雪婷龙光强马春花张广辉赵明徐祥增孟珍贵李龙根
Owner YUNNAN AGRICULTURAL UNIVERSITY
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