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Cow superovulation sustained-release agent and preparation method thereof

A slow-release agent and hyaluronic acid technology, which is applied in the direction of pharmaceutical formulations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problems affecting the efficiency of superovulation of cattle and the stress of donor cattle. Response is large, the labor cost of cattle farms increases, etc., to achieve the effect of easy operation and popularization, prolonging the action time, and improving the efficiency of super-expelling

Inactive Publication Date: 2015-07-29
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, FSH has a short half-life in cattle, about 5 hours (Laster, 1972; Demoustier et al., 1988), so frequent injections of FSH are required during superovulation to induce follicular development (Bellows et al., 1969; Monniaux et al., 1983)
For example, a conventional cattle superovulation treatment process requires intramuscular injection of FSH twice a day with an interval of 12 hours for 4 days. During the treatment process, there are problems such as a large stress response of the donor cattle, and increased labor costs for experiments and cattle farms.
In addition, with the increasing emphasis on food safety in more and more countries, estrogen (E2) is banned from the prefollicular treatment of superovulated donor cattle (Lane et al. The efficiency of counting ovulation, for example, the average number of embryos available for superovulation of American dairy cows in 2002-2011 was 6.1-6.7 (Hasler, 2012), and the improvement effect is not obvious

Method used

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  • Cow superovulation sustained-release agent and preparation method thereof
  • Cow superovulation sustained-release agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0014] Preparation of 10.0 mg / mL sustained-release agent: Weigh 100.0 mg of hyaluronic acid with an electronic balance, and dissolve 100.0 mg of hyaluronic acid in 10.0 mL of deionized water according to the concentration standard of 10.0 mg / mL. Save the settings.

[0015] Dilution of FSH: FSH is fully dissolved and diluted with 20.0mL water for injection.

[0016] Preparation of FSH-sustained release agent:

[0017] Step 1: Pre-dilution of FSH: use a 2.0mL syringe to draw 1.0mL water for injection to dilute 400.0mg of FSH, and let it stand for use after fully dissolved;

[0018] Step 2: FSH-sustained-release agent mixing: Use a 10.0mL syringe to draw 9.0mL superexhaust sustained-release agent solution, mix it with 1.0mL FSH solution to prepare 10.0ml FSH-sustained-release agent solution, and mix thoroughly in a 40°C water bath Evenly, set aside.

experiment example 1

[0020] 1. Donor cows: 15 adult dairy cows aged 40-45 months and weighing 500-550kg were selected as donors.

[0021] 2. Superovulation treatment of donor cattle

[0022] 2.1 Conventional superovulation treatment (FSH group): implant vaginal suppository (CIDR) on any day of the estrus cycle of the donor cattle (except the day of estrus) (day 0), intramuscularly inject 4.0 mL of FSH in the morning and evening of day 5; Intramuscular injection of 3mL FSH in the morning and evening of the 7th day; 2.0mL FSH in the morning and evening of the 7th day; 1.0mL FSH in the morning and evening of the 8th day. On the 7th day, FSH was injected and cloprostenol 0.6 mg and 0.4 mg were injected intramuscularly (3 and 2 tubes), respectively, and the vaginal suppository was removed on the evening of the 7th day (Table 1).

[0023] 2.2 Superovulation treatment with slow-release agent (FSH-SRF group): implant vaginal suppository (CIDR) on any day of the estrus cycle of the donor cow (except the d...

experiment example 2

[0034] 1. Donor cows: select 2 adult cows that have been used for conventional superovulation as donors

[0035] 2. The specific steps are the same as 2.2 in Experimental Example 1

[0036] 3. Results: 24 embryos were obtained from 2 donor cows, and the number of usable embryos was 7.0 per head.

[0037] The above experimental examples show that hyaluronic acid is added to FSH as a slow-release agent, which delays the release of exogenous FSH in the donor cattle, can obtain a superovulation effect equivalent to the conventional superovulation effect, and simplifies the superovulation procedure of cattle .

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Abstract

The present invention provides a novel cow superovulation sustained-release agent, which is a hyaluronic acid solution with a concentration of 5.0-10.0 mg / mL. According to the present invention, with the combination of the cow superovulation sustained-release agent and FSH, the FSH action effect is prolonged, the cow superovulation process is simplified, the superovulation efficiency is improved, and the superovulation effect equal to the conventional superovulation effect can be obtained, wherein 8 injections within 4 days is reduced to 2 injections within 4 days; and the operation of the method is simple, the superovulation efficiency is improved, and the method is easy to operate at the cow farming site and is easy to promote and use.

Description

technical field [0001] The invention relates to the field of animal husbandry, in particular to a bovine superovulation slow-release agent and a preparation method thereof. Background technique [0002] The main purpose of superovulation treatment in cattle is to induce a greater number of ovulated follicles, thereby obtaining a greater number of transferable embryos and a higher transfer pregnancy rate (Bo et al., 2002). Traditional drugs for superovulation in cattle include pregnant horse serum (PMSG) or the gonadotropin-follicle-stimulating hormone (FSH) (Mapletoft et al., 1992). A single intramuscular injection of PMSG can not only induce follicle development on the donor ovary (Schams et al., 1977), but also due to its long half-life (more than 40h), the donor bovine ovary will form large follicles during superovulation. Non-ovulated follicles affect the efficiency of embryo collection and must be used in combination with anti-PMSG in cattle superovulation (Dieleman et...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/24A61K47/36A61P15/08
Inventor 郝海生朱化彬杜卫华王栋宋金辉
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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