Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of cattle ultra-exhaustion slow-release agent and preparation method thereof

A drug, hyaluronic acid technology, applied in the field of bovine superovulation slow-release agent and its preparation, can solve the problems affecting the efficiency of bovine superovulation, the stress response of donor cattle, and the increase of labor costs in cattle farms, etc., to achieve It is easy to operate and promote the use, prolongs the action time, and improves the effect of super-exhaust efficiency

Inactive Publication Date: 2018-01-19
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, FSH has a short half-life in cattle, about 5 hours (Laster, 1972; Demoustier et al., 1988), so frequent injections of FSH are required during superovulation to induce follicular development (Bellows et al., 1969; Monniaux et al., 1983)
For example, a conventional cattle superovulation treatment process requires intramuscular injection of FSH twice a day with an interval of 12 hours for 4 days. During the treatment process, there are problems such as a large stress response of the donor cattle, and increased labor costs for experiments and cattle farms.
In addition, with the increasing emphasis on food safety in more and more countries, estrogen (E2) is banned from the prefollicular treatment of superovulated donor cattle (Lane et al. The efficiency of ovulation, for example, the average number of embryos available for superovulation of American dairy cows in 2002-2011 was 6.1-6.7 (Hasler, 2012), and the improvement effect is not obvious

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of cattle ultra-exhaustion slow-release agent and preparation method thereof
  • A kind of cattle ultra-exhaustion slow-release agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0014] Preparation of 10.0 mg / mL sustained-release agent: Weigh 100.0 mg of hyaluronic acid with an electronic balance, and dissolve 100.0 mg of hyaluronic acid in 10.0 mL of deionized water according to the concentration standard of 10.0 mg / mL. Save the settings.

[0015] Dilution of FSH: FSH is fully dissolved and diluted with 20.0mL water for injection.

[0016] Preparation of FSH-sustained release agent:

[0017] Step 1: Pre-dilution of FSH: use a 2.0mL syringe to draw 1.0mL water for injection to dilute 400.0mg of FSH, and let it stand for use after fully dissolved;

[0018] Step 2: FSH-sustained-release agent mixing: Use a 10.0mL syringe to draw 9.0mL superexhaust sustained-release agent solution, mix it with 1.0mL FSH solution to prepare 10.0ml FSH-sustained-release agent solution, and mix thoroughly in a 40°C water bath Evenly, set aside.

experiment example 1

[0020] 1. Donor cows: 15 adult dairy cows aged 40-45 months and weighing 500-550kg were selected as donors.

[0021] 2. Superovulation treatment of donor cattle

[0022] 2.1 Conventional superovulation treatment (FSH group): implant vaginal suppository (CIDR) on any day of the estrus cycle of the donor cattle (except the day of estrus) (day 0), intramuscularly inject 4.0 mL of FSH in the morning and evening of day 5; Intramuscular injection of 3mL FSH in the morning and evening of the 7th day; 2.0mL FSH in the morning and evening of the 7th day; 1.0mL FSH in the morning and evening of the 8th day. On the 7th day, FSH was injected and cloprostenol 0.6 mg and 0.4 mg were injected intramuscularly (3 and 2 tubes), respectively, and the vaginal suppository was removed on the evening of the 7th day (Table 1).

[0023] 2.2 Superovulation treatment with slow-release agent (FSH-SRF group): implant vaginal suppository (CIDR) on any day of the estrus cycle of the donor cow (except the d...

experiment example 2

[0034] 1. Donor cows: select 2 adult cows that have been used for conventional superovulation as donors

[0035] 2. The specific steps are the same as 2.2 in Experimental Example 1

[0036] 3. Results: 24 embryos were obtained from 2 donor cows, and the number of usable embryos was 7.0 per head.

[0037] The above experimental examples show that hyaluronic acid is added to FSH as a slow-release agent, which delays the release of exogenous FSH in the donor cattle, can obtain a superovulation effect equivalent to the conventional superovulation effect, and simplifies the superovulation procedure of cattle .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a novel bovine superovulation slow-release agent, which is a 5.0-10.0 mg / mL hyaluronic acid solution. The bovine superovulation slow-release agent of the present invention is used in combination with FSH, which prolongs the action time of FSH, simplifies the bovine superovulation procedure, reduces injections from 8 times in 4 days to 2 injections in 4 days, and improves the efficiency of superovulation. It can obtain the same super-drainage effect as conventional super-drainage. This method is easy to operate, improves the efficiency of super-drainage, and is convenient for on-site operation and popularization in cattle farms.

Description

technical field [0001] The invention relates to the field of animal husbandry, in particular to a bovine superovulation slow-release agent and a preparation method thereof. Background technique [0002] The main purpose of superovulation treatment in cattle is to induce a greater number of ovulated follicles, thereby obtaining a greater number of transferable embryos and a higher transfer pregnancy rate (Bo et al., 2002). Traditional drugs for superovulation in cattle include pregnant horse serum (PMSG) or the gonadotropin-follicle-stimulating hormone (FSH) (Mapletoft et al., 1992). A single intramuscular injection of PMSG can not only induce follicle development on the donor ovary (Schams et al., 1977), but also due to its long half-life (more than 40h), the donor bovine ovary will form large follicles during superovulation. Non-ovulated follicles affect the efficiency of embryo collection and must be used in combination with anti-PMSG in cattle superovulation (Dieleman et...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/24A61K47/36A61P15/08
Inventor 郝海生朱化彬杜卫华王栋宋金辉
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com