A kind of biological control method of rice blast
A technology of rice blast and extract, which is applied in the field of biological control of rice blast, can solve the problems such as the decline of control effect, and achieve the effects of low cost, reduced infection base, and wide source of raw materials
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Embodiment 1
[0029] A preparation method of water hyacinth tissue extract extract, the specific steps are as follows:
[0030] Step 1: Decompose the water hyacinth into roots, stems, leaves, flowers and seeds, dry them in an oven at 40°C for 5 hours, and crush them with a plant grinder;
[0031] Second step: Soak the pulverized objects of roots, stems, leaves, flowers and seeds of water hyacinth with organic solvent respectively, and the organic solvent is n-hexane; The temperature is 40°C; the slag is removed by filtration to obtain an extract with a mass concentration of 0.05g / mL;
[0032] Step 3: Concentrate the filtered extract under reduced pressure at a temperature of 40°C to obtain water hyacinth root extract, water hyacinth stem extract, water hyacinth leaf extract, and water hyacinth flower extract respectively. extract and water hyacinth seed extract.
Embodiment 2
[0034] A preparation method of water hyacinth tissue extract extract, the specific steps are as follows:
[0035] Step 1: Decompose the water hyacinth into roots, stems, leaves, flowers and seeds, dry them in an oven at 60°C for 12 hours, and crush them with a plant grinder;
[0036] Second step: Soak the pulverized objects of roots, stems, leaves, flowers and seeds of water hyacinth respectively in an organic solvent, the organic solvent being ethyl acetate; the soaking is carried out in 5 minutes, each soaking time is 8 hours, The soaking temperature is 60°C; filter and remove the slag to obtain an extract with a mass concentration of 0.10g / mL;
[0037] Step 3: Concentrate the filtered extract under reduced pressure at a temperature of 60°C to obtain water hyacinth root extract extract, water hyacinth stem extract extract, water hyacinth leaf extract extract, water hyacinth flower extract extract and water hyacinth seed extract.
Embodiment 3
[0039] A preparation method of water hyacinth tissue extract extract, the specific steps are as follows:
[0040] Step 1: Decompose the water hyacinth into roots, stems, leaves, flowers and seeds, dry them in an oven at 50°C for 8 hours, and crush them with a plant grinder;
[0041] Second step: Soak the root, stem, leaf, flower and seed of water hyacinth with organic solvent respectively, and the organic solvent is ethanol; The soaking is divided into 4, and the soaking time is 7 hours each time. at 50°C; remove the slag by filtration to obtain an extract with a mass concentration of 0.08g / mL;
[0042] Step 3: Concentrate the filtered extract under reduced pressure at a temperature of 50°C to obtain water hyacinth root extract, water hyacinth stem extract, water hyacinth leaf extract, and water hyacinth flower extract respectively. extract and water hyacinth seed extract.
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