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Method for micro-measurement of breast milk

A breast milk and trace technology, applied in the measurement of color/spectral characteristics, etc., can solve the problem of large amount of breast milk

Active Publication Date: 2017-12-08
泰安市康宇医疗器械有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, many hospitals, maternal and child health care centers and other institutions that need breast milk testing do not have the corresponding technology to analyze the nutrients in breast milk, especially trace elements, to provide a basis for supplementing nutrition for breastfeeding mothers and better supplement nutrition for babies, but currently there are The amount of breast milk required for breast milk analysis is 5 ml to 20 ml, and the amount of breast milk used is large

Method used

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Embodiment Construction

[0051] The method for micro-measurement of breast milk of the present invention uses the measuring principle of the instrument as follows: the qualitative and quantitative analysis of the substance is carried out by utilizing the absorption phenomenon of light of different wavelengths by the substance, which conforms to the Lambert-Beer law, T═I / I.

[0052] A═Log(I. / I)

[0053] Among them: T—transmittance

[0054] I - transmitted light intensity

[0055] I. ——incident light intensity

[0056] A——Absorptivity

[0057] Include the following steps,

[0058] (1) Instrument calibration

[0059] 1. Zinc calibration method

[0060] The main components of zinc reagent:

[0061] Zinc reagent 1: Tris buffer

[0062] Zinc Reagent 2: Zincon

[0063] Standard solution: zinc sulfate

[0064] Calibration steps:

[0065] a) Blank tube: Take 1700ul of zinc reagent 1 and 100ul of zinc reagent 2 and mix them in the test tube;

[0066] b) Standard tube: take 1500ul of zinc reagent 1 a...

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Abstract

The invention discloses a method for micro-measurement of breast milk. The method for micro-measurement of breast milk in the present invention uses the measurement principle of the instrument as follows: the qualitative and quantitative analysis of the substance is carried out by utilizing the absorption phenomenon of light of different wavelengths by the substance, which conforms to the Lambert-ratio Ear law, including the following steps, 1) Calibration of the instrument; 2) Sample centrifugation: Put the breast milk sample in a centrifuge tube for later use. Do not stand it upside down or shake it when taking it out, so as not to make the sample turbid. The disposable needle tube is inserted into the centrifuge tube from the wall of the centrifuge tube to suck out the clarified breast milk supernatant in the middle of the centrifuge tube and inject it into the test tube for later use; 3) Sample detection; method, the amount of breast milk used is small, the waste of breast milk is reduced, and the detection method is simple and convenient.

Description

technical field [0001] The invention relates to a method for micro-measurement of breast milk, which detects breast milk components in a small amount and belongs to the field of breast milk detection. Background technique [0002] Infancy is the period of the fastest growth and development of children. It is necessary to take in the right amount of nutrients to ensure normal growth and development. Breast milk contains various nutrients needed for the growth and development of newborns, which is suitable for the digestion and absorption of the gastrointestinal function of newborns. Moreover, its quality and quantity will continue to change to meet the needs of the baby's growth and development, but the nutrition of breast milk is not balanced enough to affect the baby's development. Therefore, it is necessary to analyze the nutritional composition of the main food sources of infants. [0003] At present, many hospitals, maternal and child health care centers and other insti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 徐磊
Owner 泰安市康宇医疗器械有限公司
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