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Cadmium strongly inducible promoter cds2 and its application

A promoter and induced expression technology, which is applied in the field of biotechnology and plant genetic engineering, can solve the problems of cadmium measurement interference, etc., and achieve the effect of high accuracy, high detection sensitivity and low detection limit

Active Publication Date: 2019-08-06
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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Problems solved by technology

The national standard analysis method is the atomic absorption method. Although the detection limit of this method is low, there are many coexisting ions after the soil pollution is decontaminated, which will interfere with the determination of cadmium to a certain extent, and the metal ion has the greatest interference effect.

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  • Cadmium strongly inducible promoter cds2 and its application
  • Cadmium strongly inducible promoter cds2 and its application
  • Cadmium strongly inducible promoter cds2 and its application

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Embodiment Construction

[0038] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0039] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0040] Obtaining Cd-S2 Promoter Containing Restriction Sites

[0041] 1. Plant material

[0042] The mature seeds of Nipponbare were preserved by the Biotechnology Office of the Rice Institute of Anhui Academy of Agricultural Sciences.

[0043] 2. Strains and plasmids

[0044] The Escherichia coli strain used in this study was XL1-blue; Agrobacterium tumefaciens was EHA105, which was preserved in the Biotechnology Laboratory of Rice Institute, Anhui Ac...

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Abstract

The invention discloses a plant gene sequence. The present invention isolates, clones and identifies a rice-specific gene promoter Cd S2 that is strongly induced by cadmium (Cd) from Nipponbare rice, and treats the obtained transgenic rice with cadmium (Cd). It is found through GUS chemical staining that after the treatment The transgenic plants had obvious staining in various parts. And through the RT-qPCR analysis of the expression of Gus gene before and after treatment, it was found that after cadmium (Cd) treatment, the expression of Gus gene in transgenic plants was 887.9 times that of before treatment, reaching 4 times the activity of the constitutive promoter ACTIN. The promoter can be used to reflect the intensity of cadmium pollution in soil through its expression level in rice. Its characteristics of simple operation, low detection limit, trace sensitivity and high accuracy are of great significance to the study of environmental relations and the improvement of ecological environment. , play an important role in sustainable development, and can actively analyze the principles of pollution formation, and further provide valuable experience for their avoidance and controllable transformation.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a plant cadmium-inducible expression promoter and its expression and application in rice. The promoter can drive the expression of a target gene in a plant under cadmium-induced conditions in a rice transgenic regulation system, and It can be used to determine the cadmium content in soil. Background technique [0002] With the rapid development of modern industrial and agricultural production, the industrial "three wastes", the amount of agricultural chemical fertilizers, and solid waste continue to increase, resulting in a sharp increase in the content of heavy metals in the soil. The problem of heavy metal pollution is becoming more and more serious, and has become one of the main problems worldwide (Deng Xiangxiong et al., 2009). Cadmium (Cd) is one of the most toxic heavy metals. Compared with other non-esse...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/82A01H5/00C12Q1/6895
Inventor 魏鹏程杨剑波李浩张银萍马卉李莉秦瑞英李娟
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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