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Method for detecting organophosphorus pesticide residues in fruits and vegetables

A technology for organophosphorus pesticides and pesticide residues, which is used in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials. It can solve the problems of long time, high detection cost, and complicated sample pretreatment. problem, to achieve the effect of simple operation and low cost

Inactive Publication Date: 2015-07-01
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned detection methods can only be operated under certain conditions such as a laboratory. The pre-treatment of samples by liquid phase and gas phase methods is more complicated, and the operation of large-scale instruments requires special training. The detection cost is high and the time is long.

Method used

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  • Method for detecting organophosphorus pesticide residues in fruits and vegetables
  • Method for detecting organophosphorus pesticide residues in fruits and vegetables
  • Method for detecting organophosphorus pesticide residues in fruits and vegetables

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 3 parts of blank distilled water that does not contain organophosphorus pesticides, 10mL each, and completely immerse 3 pieces of blank qualitative filter paper with a size of 3cm×0.5cm in each solution, take it out after 1min and dry it, place it on the bromine vapor for fumigation, Add 1 drop of 5% Congo red ethanol solution, and observe the color change result after the reaction is completed. The results showed that there was no difference in the color results of the three pesticide-free test strips.

Embodiment 2

[0027] Take 10mL each of 3 standard aqueous solutions containing organophosphorus pesticides with a concentration of 20mg / L, respectively immerse 3 pieces of blank qualitative filter paper with a size of 3cm×0.5cm in each solution, take them out after 1min to dry, and place them on bromine vapor After fumigation, drop 1 drop of 5% Congo red ethanol solution, and observe the color change result after the reaction is completed. The results showed that there was no difference in the color results of the three test strips containing organophosphorus pesticides, which indicated that the method had good repeatability.

Embodiment 3

[0029] Draw 0, 1, 2, 3, 4, 5mL of organophosphorus pesticide standard solutions with a concentration of 200mg / L and add them to six 10ml colorimetric tubes to distilled water to volume. Submerge 6 pieces of blank qualitative filter paper with a size of 3cm×0.5cm in each solution, take them out after 1min and dry them in the air, place them on bromine vapor for fumigation, add 1 drop of 5% Congo red ethanol solution, and wait until the reaction is over Then observe the color change result. Save the color results in a color-printed plastic seal and make a standard color comparison card, see figure 1 .

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Abstract

The invention discloses a method for detecting organophosphorus pesticide residues in fruits and vegetables, and belongs to the field of food safety detection. The method comprises the following steps: soaking blank filter paper in a solution to be detected for 1min, airing, fumigating with bromine vapour, then developing by taking 5% Congo ethanol solution as a developing reagent, and directly reading the content of organophosphorus pesticides in the solution to be detected by comparing with colours on a standard colourimetric card. The invention provides a rapid detection method for organophosphorus pesticide contents in fruits and vegetables, wherein the detectable pesticide concentration range is 0-100mg / L. The method provided by the invention has the advantages of being sensitive, rapid, simple and convenient to operate, low in cost, and the like, does not need to be operated by professionals, facilitates self-use of common people in families, and can also be used for rapid detection in industrial and commercial law enforcement, food safety and markets.

Description

technical field [0001] The invention belongs to the field of food safety detection, in particular to a method for detecting organophosphorus pesticide residues in fruits and vegetables. Background technique [0002] People in the world today tend to be green and healthy food. Fresh fruits and vegetables with low pesticide content have attracted strong attention from all walks of life, and have gradually become the development trend of green and healthy food. In recent years, the irrational use and excessive use of pesticides, and the problem of pesticide residues in agricultural products such as fruits and vegetables have become a hot issue of great concern to our society. [0003] Organophosphorus pesticides are widely used in insecticide, bactericidal and weeding of crops. They are the most widely used pesticides in my country. They are neurotoxic substances, which mainly inhibit the activity of acetylcholinease in blood and tissues, resulting in the neurotransmitter, acet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 付大友赵鑫周良芹谭文渊
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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