Method for establishing tumor multi-drug resistant cell mode and human breast cancer multi-drug resistant cell strain established by virtue of method

Abstract

The invention belongs to the field of oncobiology and relates to a method of establishing a tumor multi-drug resistant cell mode in vitro. The method comprises the step of continuously culturing a sensitive tumor cell by taking ROS as an incubation drug to enable the sensitive tumor cell to generate multi-drug resistance. Moreover, the invention further relates to a human breast cancer multi-drug resistant cell strain established by virtue of the method. The cell strain is named MCF-7 / ROS and preserved in CCTCC (China Center for Type Culture Collection), wherein the address is 430072, Wuhan University, Wuhan, China; the preservation date is 28th, January, 2015; the preservation number is CCTCC-C201520. The method provided by the invention is simple in process and stable in modeling condition, the modeling cost can be remarkably lowered, and the method has wide applicability. The MCF-7 / ROS cell strain established by the method can serve as an advantageous experimental mode for researching related mechanism, target spot exploration and screening of new drugs.

Description

technical field [0001] The invention belongs to the field of tumor biology, and in particular relates to a method for producing a mold constructing an in vitro tumor multidrug-resistant cell model by a single-machine reaction oxygen group, and also relates to a human breast cancer multidrug-resistant cell line established by the method. Background technique [0002] The construction of tumor cell models in vitro is an important experimental basis for the study of tumor occurrence, development and intervention. The choice of modeling conditions and modeling methods is the decisive factor for model construction. It is a key platform for revealing the molecular mechanism of similar tumor formation and studying tumor drug intervention. . [0003] Tumor multidrug resistance (MDR) refers to the phenomenon that tumor cells develop drug resistance after exposure to antineoplastic drugs, and also produce cross-resistance to a variety of antineoplastic drugs with different structures...

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Problems solved by technology

This construction method can simulate the basic situation of clinical MDR to a certain extent, but there are still many deficiencies, including: (1) The model design is difficult to simulate the core mechanism of clinical MDR formation: clinical anti-tumor treatment often uses drug combination, Therefore, the MDR model established by selecting only one drug may have some common features of clinical MDR, but it will also reflect a series of personalities related to the pharmacological effects of the drug, and the malignant phenotype of the drug-resistant cell line obtained has obvious Therefore, the mechanism, target and drug research using this model as the experimental object cannot represent the general situation of MDR in other situations, and even ignore the core factors of the formation of multidrug resistance, which cannot meet the needs of scientific research

(2) Disadvantages related to the experimental operation: the traditional construction method is cumbersome and complicated to operate, high in culture costs, generally long in construction time, relatively high in failure rate, there is no uniform standard in culture conditions, and the degree of drug resistance of the obtained cells varies greatly

(3) Practical limitations: MDR cell models are a commonly used and necessary platform for studying tumor multidrug resistance mechanisms and drugs. However, the types of traditional models established are very limited, and the types of MDR cell lines commonly used in the market are few and expensive. Unable to meet individual research needs

Secondly, since only one cell line induced by one drug can be obtained for each modeling, and the obtained model only has high drug resistance to this drug, and the resistance to other drugs is limited, therefore, it is used as The results obtained by the experimental subjects will have a large deviation from the clinical MDR situation

In addition, the growth rate of drug-resistant cell lines induced by anti-tumor drugs is generally slow, which also greatly limits the use of research

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