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A kind of culture medium for in vitro germination of sunflower pollen and method for measuring sunflower pollen vigor

A technology for in vitro germination and pollen viability, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, plant cells, etc., to achieve low cost, avoid economic loss, and simple preparation

Active Publication Date: 2018-04-13
河北双星种业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the medium and culture conditions for in vitro germination of sunflower pollen, and the use of the current general pollen in vitro germination medium cannot successfully germinate sunflower pollen

Method used

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  • A kind of culture medium for in vitro germination of sunflower pollen and method for measuring sunflower pollen vigor
  • A kind of culture medium for in vitro germination of sunflower pollen and method for measuring sunflower pollen vigor
  • A kind of culture medium for in vitro germination of sunflower pollen and method for measuring sunflower pollen vigor

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Experimental program
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Effect test

Embodiment 1

[0038] A kind of method for measuring sunflower pollen vigor of the present embodiment comprises the following steps:

[0039] 1. Preparation of culture medium: Accurately weigh the above-mentioned raw materials according to the formula in Table 1 and dissolve them in water to make the above-mentioned culture medium;

[0040] 2. Collect pollen: collect the pollen on the flower disc in the Hebei experimental field at 9 am, put the pollen into a centrifuge tube, and store it in the dark;

[0041] 3. Cultivate pollen: sprinkle the pollen evenly on the culture medium, and cultivate for 2 hours at 15°C;

[0042] Fourth, microscopic examination, the calculated germination rate is 72%.

Embodiment 2

[0044] A kind of method for measuring sunflower pollen vigor of the present embodiment comprises the following steps:

[0045] 1. Preparation of culture medium: Accurately weigh the above-mentioned raw materials according to the formula in Table 1 and dissolve them in water to make the above-mentioned culture medium;

[0046] 2. Collect pollen: collect the pollen on the flower disc in the Shandong experimental field at 10 am, put the pollen into a centrifuge tube, and store it in the dark;

[0047] 3. Cultivate pollen: sprinkle the pollen evenly on the culture medium, and cultivate for 1 hour at 15°C;

[0048]Fourth, microscopic examination, the calculated germination rate is 76%.

Embodiment 3

[0050] A kind of method for measuring sunflower pollen vigor of the present embodiment comprises the following steps:

[0051] 1. Preparation of culture medium: Accurately weigh the above-mentioned raw materials according to the formula in Table 1 and dissolve them in water to make the above-mentioned culture medium;

[0052] 2. Collecting pollen: collect the pollen on the flower disc in the test field in Hainan at 11 am, put the pollen into a centrifuge tube, and store it away from light;

[0053] 3. Cultivate pollen: spread the pollen evenly on the culture medium, and cultivate for 1 hour at 20°C;

[0054] Fourth, microscopic examination, the calculated germination rate is 47%.

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Abstract

The invention discloses a sunflower pollen germination medium in vitro and a method for measuring sunflower pollen vigor, and relates to a method for measuring pollen germination ability, in particular to a method for measuring sunflower pollen germination ability. The purpose is to provide an optimum sunflower pollen germination medium in vitro and an efficient, fast and simple method for measuring sunflower pollen vigor. The medium for in vitro germination of sunflower pollen of the present invention comprises sucrose, boric acid, CaCl2 2H2O, PEG4000, MgSO4 7H2O, KNO3, KH2PO4, KCl, Na2EDTA, FeSO4 7H2O, Na2MoO4 2H2O, CuSO4 5H2O, CoCl2 6H2O, VB1 and VB6. The method for measuring sunflower pollen vigor of the present invention comprises: preparing medium; collecting pollen; cultivating pollen; and microscopic examination. The preparation of the culture medium of the invention is simple and the cost is low; the method for measuring the vigor of sunflower pollen is simple and easy to operate. The invention is used in the field of determination of sunflower pollen germination ability.

Description

technical field [0001] The invention relates to a method for measuring pollen germination ability, in particular to a method for measuring sunflower pollen germination ability. Background technique [0002] Sunflower originated in North America. It is a plant of Compositae and Helianthus. The seeds of sunflower have high economic value. Not only can they be made into popular sunflower seeds, but also high-grade edible sunflower oil with low cholesterol can be squeezed out. Sunflower has been cultivated in my country for decades. It is one of the important economic crops in more than 20 provinces, municipalities and autonomous regions. [0003] Sunflower has obvious heterosis, and most of the sunflower seeds used in production are the first generation of hybrids. The vigor of sunflower pollen is easily affected by many internal and external factors. How to identify and collect high-purity and high-viability pollen plays a vital role in ensuring the yield and quality of hybr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04C12Q1/02
Inventor 范冬冬党继革代云志李涛吴涛赵翠媛高雪萍张慧
Owner 河北双星种业股份有限公司
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