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BMP-loaded silk fibroin/collagen scaffold material and preparation method thereof

A collagen and silk fibroin technology, applied in medical science, prosthesis, etc., to solve the effect of short half-life, prolong function, and prolong full play

Inactive Publication Date: 2017-01-25
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiency of existing single collagen component tissue repair materials, provide a kind of BMP-loaded silk fibroin / collagen protein scaffold material and its preparation method

Method used

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  • BMP-loaded silk fibroin/collagen scaffold material and preparation method thereof
  • BMP-loaded silk fibroin/collagen scaffold material and preparation method thereof
  • BMP-loaded silk fibroin/collagen scaffold material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Preparation of loose layer silk fibroin / collagen film and dense layer silk fibroin / collagen film

[0026] 1. Preparation of silk fibroin

[0027] Cut silkworm cocoons into pieces and add 0.5% Na 2 CO 3 solution, in a constant temperature water bath at 98±2°C for 30 min, twice in total. After taking it out, wash it twice with boiling water, and then wash it 8 times with deionized water. Dry in an oven at 60°C to obtain dry silk fibroin. Weigh the silk fibroin silk after silk degumming, use CaCl 2 / H 2 O / C 2 h 5 OH solution (molar ratio 1:8:2) was dissolved in a constant temperature water bath at (78±2)°C, and the bath ratio was 1:25. Filter with gauze to obtain the silk fibroin stock solution. Dialyze with a fibrous semipermeable membrane dialysis bag with a molecular weight cut-off of 8000 D-14000 D for 3 days to remove calcium chloride and ethanol. Filter to obtain silk fibroin solution.

[0028] 2. Collagen preparation

[0029] Cut the fish skin...

Embodiment 2

[0035] Embodiment 2: Preparation of microspheres loaded with BMP-2

[0036]Weigh 0.25 g of PLGA, dissolve in 2.5 mL of dichloromethane, and set aside in an ice bath. Weigh 100 uL of 2% BMP-2 solution, emulsify for 2 min at 5000-15000 r / min to form colostrum, immediately pour 25 mL of 0.5% PVA solution, and pass through a homogenizer at 8000 r / min Stir for 1 min to form double emulsion. Magnetically stirred for 4 h to evaporate the organic solvent. The double emulsion was centrifuged at 8000 rpm for 8 min at a constant temperature of 4°C, washed 5 times with deionized water, and freeze-dried to obtain PLGA microspheres loaded with BMP-2 ( figure 1 ). The particle size distribution and potential distribution of PLGA microspheres were normal, and the particle size and Zeta potential distribution of most microspheres were concentrated. Average particle size: 1233nm, average Zata potential: -29.7mV.

Embodiment 3

[0037] Example 3: Preparation of BMP-loaded silk fibroin / collagen scaffold material

[0038] Wet the cross-linked loose layer silk fibroin / collagen membrane prepared in Example 1 with double-distilled water and spread it flat, and add the aqueous solution of PLGA microspheres loaded with BMP-2 prepared in Example 2 to the surface at 4-8 °C Air-dry until there is no flowing liquid on the surface, then wet the cross-linked dense layer silk fibroin / collagen film and spread it on the upper surface of the cross-linked loose layer silk fibroin / collagen film, and air-dry at 4-8°C to obtain BMP-2-loaded silk Vegetarian protein / collagen scaffold material ( figure 2 ).

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Abstract

The invention discloses a BMP loaded silk fibroin / collagen scaffold material. The material is prepared with collagen, BMP, silk fibroin and PLGA as raw materials; a preparation method comprises the steps of preparation of a loose-layer silk fibroin / collagen membrane and a dense-layer silk fibroin / collagen membrane, preparation of BMP loaded PLGA microspheres, composite of the silk fibroin / collagen membranes and the microspheres and the like. A scaffold is a tissue engineering scaffold having no toxic or side effect on bodies and having good biocompatibility and tissue repair capacity. Based on combination of respective advantages of good biological compatibility, biological degradation and the like of two different natural biological macromolecules of silk fibroin and collagen, the BMP is introduced to the scaffold, a directional controlled-release slow-release technology is adopted, the BMP is allowed to be released persistently, the problems of relatively short in-vivo half-life period of the BMP, growth factor directional introduction local defect repairing and the like are solved effectively, and full playing of functions are prolonged.

Description

technical field [0001] The invention relates to a BMP-loaded silk fibroin / collagen scaffold material and a preparation method thereof. Background technique [0002] Collagen is the main component of the extracellular matrix (ECM), accounting for about 1 / 3 of the total protein mass in mammals, and exists in large quantities in connective tissues or organs such as skin, ligaments, cartilage, and tendons. The unique triple helix structure of collagen endows it with good biological properties, such as high tensile strength, biodegradability, low antigenic activity, low irritation and low cytotoxicity, and the ability to promote cell growth, all of which make it a An ideal biomedical material and drug carrier. However, with the development of tissue engineering, three-dimensional scaffold materials composed of single-component collagen cannot fully meet the overall requirements of tissue engineering for scaffold materials. Therefore, applying the principles and methods of compo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/54A61L27/26A61L27/18A61L27/50
Inventor 张其清王建华程娘梅杨秋张志华
Owner FUZHOU UNIV
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