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Application of radix ginseng in inhibition of ras proto-oncogene overexpression

A proto-oncogene and overexpression technology, which is applied in the direction of plant raw materials, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., can solve the problem of ginseng that has not been reported in the literature, and achieve the goal of broadening the scope of drug use and good The effect of treatment

Inactive Publication Date: 2015-06-17
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, no literature has reported that ginseng can inhibit the overexpression of the ras proto-oncogene, and there is no drug on the market that uses ginseng as a raw material, which can specifically inhibit the tumor caused by the overexpression of the ras proto-oncogene

Method used

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  • Application of radix ginseng in inhibition of ras proto-oncogene overexpression
  • Application of radix ginseng in inhibition of ras proto-oncogene overexpression
  • Application of radix ginseng in inhibition of ras proto-oncogene overexpression

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] The preparation of embodiment one ginseng medicinal liquid

[0012] Ginseng source: purchased from Lanzhou Yellow River Medicine Market.

[0013] Weigh 9 g of ginseng; first soak in distilled water for 20 minutes, then boil on low heat, decoct for 30 minutes, constant volume, centrifuge, discard the precipitate, filter the supernatant aseptically, put it in a 4°C refrigerator for later use, and dilute to different concentrations when used.

Embodiment 2

[0014] Example The effect of Ershen liquid on Caenorhabditis elegans MT2124 (let-60 hyperactivation mutation)

[0015] Ras is a small molecular protein that participates in the regulation of many important links in the development of animals. The ras signaling pathway is highly conserved from nematodes to humans. The let-60 gene encodes a conserved Ras protein in Caenorhabditis elegans, which has 83% homology with human Ras proteins. If the ras mutation, in Caenorhabditis elegans, it will cause the nematode to produce polyvagina, and in humans, it will cause the malignant proliferation of cells, leading to the occurrence of tumors. Therefore, the model organism Caenorhabditis elegans ( Caenorhabditis elegans ) Ras / MAPK signaling pathway overactivation mutants are widely used as tumor models to screen anti-tumor drugs that inhibit the overactivation of ras proto-oncogene pathway.

[0016] MT2124 is a let-60 / ras overactivated C. elegans that produces a polyvaginal phenotype. ...

Embodiment 3

[0034] Example 3 The effect of ginseng on Caenorhabditis elegans MT8189 (lin-15 mutation inactivation)

[0035] Experimental materials: Caenorhabditis elegans MT8189, polyvulva phenotype, genotype: lin-15b(n765)X, purchased from CGC (Caenorhabditis Genetics Center); Escherichia coli OP50 (uracil leaky mutant strain), used as Caenorhabditis elegans Nematode food was purchased from CGC (Caenorhabditis Genetics Center).

[0036] Concrete experimental procedure: with embodiment two.

[0037] Table 2 Effect of ginseng on Caenorhabditis elegans MT8189 (lin-15 mutation inactivation)

[0038]

[0039] In Caenorhabditis elegans, lin-15(n765)X is located upstream of ras and normally inhibits the expression of ras gene. The inactivation of this gene in the MT8189 nematode strain leads to the phenotype of multiple ovipositors in the nematode.

[0040]As shown in the table, ginseng cannot inhibit the polyvulva phenotype of lin-15(n765)X. According to the principle of genetic epistasis...

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Abstract

The invention relates to an application of radix ginseng in inhibition of ras proto-oncogene overexpression, and specifically, relates to the application of radix ginseng in preparation of anti-tumor drugs for inhibiting ras proto-oncogene overexpression. Researches indicate that about 90% of pancreatic cancer, 50% of colon cancer, 30%-40% of lung adenocarcinoma and 5%-40% of leukemia are caused by the ras proto-oncogene overexpression, and a Ras protein becomes a universally accepted target for screening drugs resistant to related malignant tumors. Animal experiments indicate that radix ginseng can specifically down-regulate overexpression caused after ras proto-oncogene mutation, but does not act on wild-type ras proto-oncogenes, so that the toxicity is low. The invention provides the new use of ginseng, and radix ginseng can be applied in preparation of the anti-tumor (tumors caused by the ras proto-oncogene overexpression) drugs.

Description

technical field [0001] The invention specifically relates to the application of ginseng in inhibiting the overexpression of ras proto-oncogene, and belongs to the field of traditional Chinese medicine. Background technique [0002] Araliaceae ginseng Panax ginseng C. A, Mey. The dried roots of Ginseng, the main active ingredients are ginsenosides and ginseng polysaccharides. It is a rare medicinal material and also a relatively common drug. It has a long history of medicinal use in my country. The clinical verification of traditional Chinese medicine shows that the main effects of ginseng include nourishing vitality, nourishing the spleen and lungs, promoting body fluid and calming the nerves, improving intelligence, anti-fatigue, anti-shock, and lowering blood lipids. [0003] About 30% of human tumors are caused by the overactivation of Ras proto-oncogene after mutation, resulting in increased expression of Ras protein. If the Ras protein is continuously activated, it ca...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61P35/00
Inventor 李红玉李孟惠万凤奇支德娟李洋黄坚张占欣陈晓雨李莹辉
Owner LANZHOU UNIVERSITY
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