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Marker site Satt557 related to soybean hundred-grain weight hereditary characteristic and application thereof

A technology for marking loci and genetic characteristics, applied in the field of soybean genetics, can solve the problems of limited means and methods of selection, and achieve the effect of shortening the breeding cycle and improving the efficiency of breeding

Inactive Publication Date: 2015-06-10
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently few reports on the marker loci that can be used for parental screening in the soybean breeding process, and the means and methods for selecting the genetic background of the 100-seed weight of the parents or hybrid offspring in the early stage of breeding are still relatively limited.

Method used

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  • Marker site Satt557 related to soybean hundred-grain weight hereditary characteristic and application thereof
  • Marker site Satt557 related to soybean hundred-grain weight hereditary characteristic and application thereof
  • Marker site Satt557 related to soybean hundred-grain weight hereditary characteristic and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Selection of germplasm resources

[0034] Selected 251 soybean germplasm resources, based on the QTL mapping of 100-seed weight of soybean and meta-analysis results of QTL of 100-seed weight, identified 30 SSR marker loci related to 100-seed weight, and verified the 30 marker loci in the resources. Banding characteristics of PCR products to detect the correlation between different banding patterns and 100-seed weight. Further verify the relationship between the marker band type and 100-seed weight through genetic population. Statistics and experiments prove that when the PCR product of Satt557 is 520bp, the soybean germplasm contains a genetic background that reduces 100-seed weight. The selected 251 soybean germplasm resources are shown in the following table:

[0035] Table 1 Names of 251 soybean germplasm resources

[0036]

[0037]

[0038]

[0039] 2. Main experimental methods

[0040] 1 Reagents used in the experiment

[0041] 1) Reagents for extracting soybean genom...

Embodiment 2

[0069] Use tools-Meta-analysis in BioMercator2.1 software to analyze the QTL clusters of each linkage group. Among the five analysis models, the model with the smallest AIC value is selected to determine a "general QTL". After Meta analysis, 15 “universal QTLs” with 100-seed weight and their interval markers were obtained, mainly located on LG B1, B2, C1, C2, D2, E, H, I, K, M and O. The genetic contribution rate is in Between 4.76%-20.82%, the maximum confidence interval is 13.62cM, and the minimum confidence interval is 1.52cM. Refer to Table 2. figure 1 . The marker Satt557 is located on linkage group C2 ( figure 2 ).

[0070] Table 2 Meta-analysis results of 100-seed weight QTLs of soybean

[0071]

[0072]

Embodiment 3

[0074] Chi-square analysis was used to analyze the significance of the phenotypic data. With alpha=0.01, 0.025, 0.05 and 0.1 as the stratification threshold, the markers obtained from the Meta analysis were divided into five levels. When the test P value was less than 0.01, the The site is a major site, and when the P value is greater than 0.1, it is considered a minor site, see image 3 . Obtained 5 key alleles at the 0.01 level: Satt541-2, Sat_145-3, Satt277-4, Satt318-2 and Satt557-3. 6 key alleles at the 0.025 level: Satt590-1, Satt242- 3. Satt318-3, Sat_355-2, Satt557-4, Sat_355-3. 6 key allele loci at the 0.05 level: Satt201-1, Satt190-2, Satt458-3, Sat_355-7, Satt161-2, Satt653 -4. 0.1 level alleles 3: Satt197-4, Satt083-10, Satt653-2. The rest are minor genes with small genetic effects below the 0.1 level, image 3 The significance of alleles for markers not listed in is 0. The T-test has 15 alleles with a P-value less than 0.05.

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Abstract

The invention discloses a marker site Satt557 related to a soybean hundred-grain weight hereditary characteristic and application thereof, and belongs to the technical field of soybean heredity. A special primer is positioned on a soybean C2 linkage group, a tight linked marker is SSR primer Satt557, and the nucleotide sequence of the primer is as shown in SEQ ID NO.1 and SEQ ID NO.2. The marker site of the primer is the Satt557 and has greatly outstanding relevance with a soybean hundred-grain weight character. The fragment of Satt557-4 (520bp) for marking a PCR product is a main effective site for influencing hundred-grain weight. The marker site Satt557 disclosed by the invention can be used for detecting the breeding group family and the soybean germplasm resources by utilizing rightly linked molecular markers, proving that the fragment is the main effective site by carrying out mathematical statistic analysis, predicting the influence of the fragment on the hundred-grain weight character and greatly improving the selection efficiency of soybean high-yield breeding.

Description

Technical field [0001] The invention relates to a marker site Satt557 related to soybean 100-seed weight genetic characteristics and its application, and belongs to the technical field of soybean genetics. Background technique [0002] Among the major food crops in my country, only soybeans have a yield lower than the world average, which is about 80% of the world’s average soybean yield. The soybean yield per mu in my country is about 115kg per mu, and the yield per mu in the United States and Brazil can reach 180kg. The breeding of soybean varieties mainly relies on the professional experience of the breeder, and the later screening work cycle is long. In particular, the molecular marker research on soybean 100-seed weight is still at a relatively elementary level and cannot be applied to breeding practice. Therefore, increasing soybean yield is of great significance to my country's soybean production. [0003] Controlling the proper 100-seed weight of soybeans is an effective...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 陈庆山刘春燕辛大伟朱荣胜胡振帮蒋洪蔚齐照明孙亚男韩雪
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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