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Tissue culture and rapid propagation method for lobularia maritima

A tissue culture fast propagation and fragrant snowball technology, applied in the field of plant tissue culture, can solve problems such as rot, difficulty in collecting seeds, and cuttings are easily infected by pathogens, and achieve the effect of promoting commercialization and enhancing reproduction coefficient.

Inactive Publication Date: 2015-06-03
黎有辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The alyssum is propagated by seeds, the seeds are flat and small, and it is difficult to collect the seeds, and the widespread planting is limited by the season; the alyssum can also be propagated by cuttings, but the cuttings are easily infected by pathogens and rot

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Obtaining sterile seedlings: select full-bodied seeds of Aurantium aurantium, first soak them in deionized water for 3 hours, filter the water, disinfect them with 75% alcohol on an ultra-clean workbench for 30 seconds, then disinfect them with 0.1% mercuric chloride solution for 8 minutes, and finally Rinse 3 times with sterile water. After disinfection, the seeds were inoculated in the germination medium for seed germination culture. After inoculation, it was placed under the condition of 12 hours of light per day, the light intensity was 2000lx, the culture temperature was 25°C, and the relative air humidity was 75% and the germination rate reached 96% after 30 days of cultivation. The germination medium is: 1 / 2MS+0.02mg / L NAA+15g / L sucrose+3.5g / L agar, with a pH of 5.4.

[0019] (2) Induction culture: inoculate the stem segment of about 1 cm of sterile seedlings on the induction medium for induction culture. After inoculation, it was placed in the light fo...

Embodiment 2

[0024] (1) Acquisition of sterile seedlings: select the plump seeds of Snowball, soak them in deionized water for 5 hours, filter out the water, disinfect them with 75% alcohol on the ultra-clean workbench for 40 seconds, then disinfect them with 0.1% mercuric chloride solution for 10 minutes, and finally Rinse 3 times with sterile water. After disinfection, the seeds were inoculated in the germination medium for seed germination culture. After inoculation, it is placed under the conditions of 12 hours of light per day, the light intensity is 2000lx, the culture temperature is 27°C, and the relative air humidity is 75% and the germination rate reaches 100% after 30 days of cultivation. The germination medium is: 1 / 2MS+0.05mg / L NAA+15g / L sucrose+3.5g / L agar, with a pH of 5.7.

[0025] (2) Induction culture: inoculate the stem segment of about 1 cm of sterile seedlings on the induction medium for induction culture. After inoculation, it was placed in the light for 14 hours...

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Abstract

The invention discloses a tissue culture and rapid propagation method for lobularia maritima. The lobularia maritima belongs to cruciferae lobularia perennial herb or subshrub plant, is short in plant and more in branches, is high-quality flower and plant for decorating a rock garden, can be used as a potted plant, a ground cover and the like, and also has a relatively good adsorption effect on heavy metal Ni. Through plant in-vitro culture, a large number of high-quality seedlings can be obtained within a short time, the propagation coefficient is greatly increased, the limitations on environmental conditions of seasons are avoided, and the defects in conventional propagation are made up through the propagation mode. A lobularia maritima seed is taken as an explant, a lobularia maritima in-vitro replanting plant is successfully obtained by adventive bud induction culture, multiplication culture, rooting culture, acclimatization and transplantation, and the like, a lobularia maritima tissue culture and rapid propagation system is established, and commercial and large-scale development of the lobularia maritima is promoted.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of snowballs. Background technique [0002] Fragrant Snowball ( Lobularia maritima ), also known as Ting mustard and small white flower, is a perennial herb or semi-shrub plant of the genus Cauliflower in the family Brassicaceae. Snow balls are native to the Mediterranean coast and are cultivated in parks and flower gardens in Hebei, Shanxi, Jiangsu, Zhejiang, Shaanxi, Xinjiang and other provinces in China. There are 5 kinds of flower colors, the flowers bloom like a blanket, the fragrance is fresh and elegant, and it is drought-resistant. It is a beautiful rock garden flower, and can also be used as a ground cover, flower bed, border plant on the edge of a flower border, potted plant or as a window sill flower. The fragrant snow ball can replace the lawn to cover the ground, prevent soil erosio...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 黎有辉
Owner 黎有辉
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