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Method for screening and annotating of longissimus dorsi differential expression genes of pigs of different varieties

A technology for differentially expressed genes and longissimus dorsi of pigs, which can be used in special data processing applications, instruments, electrical digital data processing, etc., and can solve problems such as difficulty and inability

Inactive Publication Date: 2015-05-20
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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Problems solved by technology

Due to the complexity of the formation of meat quality traits and the inability (or difficulty) to measure them in vivo, it is difficult for traditional breeding methods to make great genetic progress in meat quality traits

Method used

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  • Method for screening and annotating of longissimus dorsi differential expression genes of pigs of different varieties
  • Method for screening and annotating of longissimus dorsi differential expression genes of pigs of different varieties
  • Method for screening and annotating of longissimus dorsi differential expression genes of pigs of different varieties

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Experimental program
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Embodiment Construction

[0018] 1 Materials and methods

[0019] 1.1 Test materials and sequencing

[0020] The tissue samples required for the experiment came from two different pig breeds, Wannan Huahua pig and Yorkshire pig. Adult longissimus dorsi muscle tissue was collected. Three individuals were collected from each pig breed. After the samples were collected, they were stored at -80°C. The total RNA of the sample was sent to Shanghai Bohao Biotechnology Co., Ltd. for sequencing using Solexa sequencing technology.

[0021] 1.2 Data analysis

[0022] 1.2.1 Raw data processing.

[0023] The raw image data generated by the sequencer is converted into sequence data by Base Calling, which we call raw sequence data, and the results are stored in the FASTQ file format. Since the original sequence data may contain low-quality sequences, linker sequences and other impurity sequence data, it cannot be directly used for bioinformatics analysis, so the original sequence data must be converted into high-q...

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Abstract

The invention discloses a method for screening and annotating of longissimus dorsi differential expression genes of pigs of different varieties. The method comprises the following steps that 1, sampling and sequencing are conducted; 2, original data are processed; 3, differential genes are screened; 4, function significance enrichment analysis is conducted; 5, Pathway significance enrichment analysis is conducted. According to the method, a high throughout transcriptome sequencing technology is adopted, the gene expression profiles of longissimus dorsi tissue of the pigs of different varieties are established, the differential expression genes are obtained, the differential expression genes are selected through GO analysis and pathway analysis, the key gene of controlling the meat quality trait is determined, control over muscle growth and development and fat metabolism can be better understood, and the molecular biology evidence is provided for the following research of the molecular mechanism forming the difference of the meat quality and the growth trait of domestic and oversea varieties.

Description

technical field [0001] The invention relates to a method for screening and annotating differentially expressed genes in longissimus dorsi of different breeds of pigs. Background technique [0002] Pork is the main source of human animal protein, and my country is currently the largest country in the world for pig production and pork consumption. Improving meat quality has become a key factor in the pork industry and in meeting consumer taste, health and nutritional needs. Skeletal muscle is the most abundant tissue in the animal body, accounting for about 40% of the animal's body weight. The difference in the growth and development of pig muscle is an important reason for the difference in pork quality between different breeds. Due to the complexity of the formation of meat quality traits and the inability (or difficulty) to measure them in vivo, it is difficult for traditional breeding methods to make great genetic progress in meat quality traits. Therefore, the use of mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/20
Inventor 王重龙李小金钱坤刘林清钱蓉
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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