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Alpha LISA detection kit for zeranol and analogue in meat product

A technology of zearalenol and zearalenol, which is applied in the field of AlphaLISA detection kits for zearalenol and its analogs in meat products, can solve the problems of high personnel requirements, time-consuming, complicated operation and the like

Inactive Publication Date: 2015-05-20
INSPECTION & QUARANTINE TECH CENT OF CHONGQING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing detection methods for zearalanol and its analogs include liquid chromatography-tandem mass spectrometry, gas chromatography-mass spectrometry, etc., but these methods have expensive equipment, cumbersome operations, time-consuming, and high personnel requirements. insufficient

Method used

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  • Alpha LISA detection kit for zeranol and analogue in meat product
  • Alpha LISA detection kit for zeranol and analogue in meat product
  • Alpha LISA detection kit for zeranol and analogue in meat product

Examples

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Embodiment Construction

[0012] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0013] The anti-zearalanol monoclonal antibody in the kit can be purchased from the market, or can be prepared by conventional methods for preparing monoclonal antibodies. Streptavidin donor magnetic beads include 100 μg / mL donor magnetic beads, 25 mM HEPES, 100 mM NaCl solution and 0.05% Proclin-300. The detection buffer is selected from the buffer in the conventional detection kit.

[0014] 1. Antigen purification

[0015] Step 1: Column Treatment

[0016] 1. Remove the sealing cap at the bottom of the desalting column, and loosen the top cap (do not remove the top cap);

[0017] 2. Put the column into a 1.5-2mL centrifuge tube. Cen...

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Abstract

The invention discloses an Alpha LISA detection method for zeranol and an analogue in a meat product and an Alpha LISA detection kit. The detection kit includes a zeranol standard substance, a beta-zeranol standard substance, an alpha-zearalanel standard substance, a beta-zearalanel standard substance, a zearalenone standard substance, a zearalenone standard substance, biotinylated zeranol, an monoclonal antibody resisting zeranol, an anti-mouse IgG coupled receptor magnetic bead, a streptavidin donor magnetic bead, a detection buffer solution, a white reaction plate, a sealing film and an operating manual. According to the detection method and the detection kit, the amount of samples is few, the sensitivity is high, no washing steps are needed and the detection period is short.

Description

technical field [0001] The present invention relates to the detection of zearalenol, β-zearalenol, α-zearalenol, β-zearalenol, zearalenone and zearalenone in meat products, specifically The invention relates to an AlphaLISA detection method and kit for zearalanol and its analogues in meat products. Background technique [0002] The residue of toxic metabolites in meat products has attracted more and more attention from the international community. In order to maximize the benefits, some unscrupulous traders illegally add zearalanol and other hormone drugs to animal feed to promote protein synthesis in animals. , improve feed utilization, thereby producing a role in promoting weight gain and obtaining more economic returns. Zearalanol and its metabolites have the biological activity of estrogens, and have inhibitory effects on gonadotropin-binding receptors and liver hormone-binding receptors in vitro. Residues of estrogens can cause dysfunction of human sex hormones and af...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/74
Inventor 王国民聂福平郗存显王雄李贤良杨俊
Owner INSPECTION & QUARANTINE TECH CENT OF CHONGQING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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