Method for separating rat hepatocytes
A separation method and liver cell technology, applied in the field of rat liver cell separation, can solve the problems of large consumption and expensive collagenase, and achieve the effects of low cost, high utilization of collagenase, and simple operation
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[0021] In order to make the object, technical solution and advantages of the present invention clearer, the following examples further describe the present invention in detail. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0022] The invention takes SD rats, male, with a body mass of 200±20 g as objects, and separates rat liver cells. The specific operation is as follows:
[0023] Step 1, taking out the rat liver;
[0024] Rats were injected with heparin sodium, intraperitoneally anesthetized with pentobarbital sodium, disinfected with 75% ethanol, and opened the abdominal cavity; the intravenous indwelling needle was inserted into the portal vein and ligated, and the superior and inferior hepatic vena cava were ligated with surgical thread.
[0025] Step 2. Preheat the calcium-free perfusate at 35~38℃ and perfuse until the liver expands;
[0026] The perfusion flow ...
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