Large-scale production method for B5 (containing carbon source) solid culture medium

Abstract

The invention discloses a large-scale production method for a B5 (containing a carbon source) solid culture medium, belonging to the field of plant biotechnologies. The large-scale production method comprises the following steps: firstly, calculating the demanded quantities of 18 elements contained in the B5 (containing the carbon source) solid culture medium after 1000 times of amplification; accurately weighing the amount of the amplified medicine, wherein CuSO4.5H2O and CoCl2.6H2O need to be accurately weighed by a one over ten-thousand balance; sequentially mixing the 18 elements according to the demanded quantities, namely, mixing the two fewest elements at first, and then adding the second fewest elements and uniformly mixing, and the like; sealing per 250g of the solid culture medium in a solid plastic container; and unsealing for use as needed respectively. The B5 (containing the carbon source) solid culture medium after 1000 times of amplification can be prepared through the method, has the characteristics of being convenient to operate, rapid, simple to apply, and having small errors of different batches and in the batches, avoids the phenomena of large errors, precipitation, mildewing and the like caused by the influences of manual operations of calculation, weighing and the like, and external factors such as weather, temperature and water content during preparation for a B5 liquid culture medium, as well as can be applied to biotechnological application of plant tissue culture and the like.

Description

technical field [0001] The invention discloses a large-scale production method of a B5 (containing carbon source) solid culture substrate, which belongs to the field of plant biotechnology. More specifically, it is a five-step process including accurate calculation, accurate weighing, mixing less and more, sealing, and unsealing, in order to obtain culture substrates with minimal errors between different batches and batches, and reduce Method of trial and error. Background technique [0002] Plant tissue culture technology or soilless culture technology usually requires the preparation of the mother liquid of the medium, that is, the preparation of a liquid that is enlarged by a certain multiple and contains various types of reagents. However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four categories are dif...

AI Technical Summary

Problems solved by technology

However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four categories are different, resulting in inconsistent quality of medicines, and one is prone to calculation errors; Two, the manufacturer and quality of the medicine are inconsistent, even if the calculation and weighing are consistent, it will affect the test results; three, because the mother liquor is in a liquid state, at the turn of spring and summer, due to the increase in temperature and humidity, it is extremely easy to cause mother liquor infection Bacteria and mold cause different colors or precipitation in the mother liquor, which wastes raw materials; four will cause large errors due to the difference in the scale of the container (especially trace elements) and the precision of the instrument.

[0003] In view of the above-mentioned shortcomings of mother liquor preparation, the preparation of culture medium with conventional mother liquor often leads to failure or unrepeatable test results due to factors such as weighers, drugs, seasons, etc., which greatly affects the use of plant tissue culture or culture media. The process of carrying out trials of soilless cultivation technology has hit the confidence of the experimenters