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A method for constructing the dominant flora of mutual fatty acid oxidizing bacteria and electrogenic bacteria

A technology of dominant bacterial flora and construction method, applied in the field of sewage biological treatment and its reuse, can solve the problems of unbalanced metabolism and transformation of intermediate products, limited organic matter removal capacity, harsh growth environment of strains, etc., to maintain the stability of anaerobic system , Simple structure, stable operation

Active Publication Date: 2017-06-16
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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  • Abstract
  • Description
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Problems solved by technology

Through the study of ecological factors such as pH, ORP (oxidation-reduction potential) and alkalinity, find out the most suitable range of ecological factors for co-culture, and improve the treatment efficiency by adjusting the most suitable parameters in engineering, but it is still not possible Solve the problem of unbalanced metabolism and transformation of intermediate products; acid-resistant methanogens that can be isolated and cultivated are currently limited, and these strains have harsh growth environments, poor adaptability to the external environment, and limited ability to remove organic matter. None of these can fundamentally solve the problem of reactor acidification

Method used

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Embodiment Construction

[0016] The present invention will be described in detail below based on specific embodiments. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0017] 1. Enrichment of electrogenic bacteria

[0018] The enrichment of electrogenic bacteria adopts the method of MFC:

[0019] Anode material: carbon cloth (length × width is 4 × 2cm)

[0020] Preparation of catalytic cathode: The two sides of the stainless steel mesh of the cathode are used as the diffusion layer and the catalytic layer. On the diffusion layer, PTFE (polytetrafluoroethylene, wt60%) is evenly coated on the stainless steel mesh, and placed in a muffle furnace at 350 ° C for burning 10 min, repeat the above steps 3 times;

[0021] On the catalytic layer, dissolve 16mg of nickel nanoparticles in 13.28uL of deionized water, add 53.2...

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Abstract

The invention discloses a construction method of dominant bacterial community of syntrophism fatty acid oxidizing bacteria and current-producing bacteria. The syntrophism fatty acid oxidizing bacteria are metabolized by virtue of a volatile organic fatty acid as a substrate; and the fermented tail end product acetic acid is further utilized by the current-producing bacteria under the catalysis of external voltage, so that the gradient degradation of organic matters is achieved; the treatment efficiency of high-concentration organic waste water and the stability of the system are improved; meanwhile, the productive rate of hydrogen is increased; unified pollution control and capacity is reached; the construction method comprises the following steps: gathering the syntrophism fatty acid oxidizing bacteria; gathering the current-producing bacteria; and adding two gathered success bacterial communities to an MEC reactor to remove the volatile organic fatty acids. The construction method has the advantages of being simple in structure, flexible to operate, stable to run, free of basicity adjustment, low in operation cost and the like.

Description

technical field [0001] The invention relates to the field of sewage biological treatment and its reuse, in particular to a method for constructing a dominant bacterial group of interoperating fatty acid oxidizing bacteria and electrogenic bacteria. Background technique [0002] Anaerobic biological treatment technology is widely used in the treatment of high-concentration organic wastewater due to its economy, safety and environmental protection, and has gradually become the preferred process. [0003] In the traditional sense, the anaerobic degradation process of macromolecular organic matter is completed step by step under the cooperation of different microbial groups such as acid-fermenting bacteria, intertrophic fatty acid oxidizing bacteria and methanogenic bacteria. These microbial groups are a "biological chain" relationship in the trophic niche: acidogenic fermentative bacteria provide volatile acids (VFAs) as substrates available to mutual fatty acid oxidizers, meta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H01M8/16
CPCY02E60/50
Inventor 朱葛夫张净瑞刘超翔
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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