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Cultivated rice hybrid infertility gene S1 and application thereof

A gene and hybrid technology, which is applied to the interspecific hybrid sterility gene S1 of cultivated rice and its application field, can solve the problems that hinder the research and development of the S1 gene hybrid sterility relationship, and there is no relevant report on the location and sequence of the S1 gene.

Inactive Publication Date: 2015-04-29
LINGNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, due to various problems, the most basic location and sequence of the S1 gene have not been reported, which seriously hinders the research and development of the relationship between the S1 gene and interspecific hybrid sterility in cultivated rice.

Method used

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  • Cultivated rice hybrid infertility gene S1 and application thereof
  • Cultivated rice hybrid infertility gene S1 and application thereof
  • Cultivated rice hybrid infertility gene S1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Acquisition of near-isogenic lines and construction of mapping populations

[0051] 1. The main test materials are the japonica recurrent parent variety IRAT216 and the near-isogenic line IRAT216S1

[0052] (1) The near-isogenic line IRAT216S1 was bred by crossing japonica IRAT216 with African cultivated rice IRGC102203, and then using IRAT216 as the recurrent parent for more than 9 consecutive backcross generations. There are three types of replacement fragments with different sizes but overlapping, and the genetic characteristics and phenotypes are very similar The near isogenic lines were named IRAT216S1-g1, IRAT216S1-g2, IRAT216S1-g3 respectively, all of which contained a single hybrid sterile S1 locus.

[0053] (2) IRAT216S1-1, IRAT216S1-2, IRAT216S1-3 were backcrossed with the recurrent parent IRAT216 and then selfed, and two BCs were obtained respectively 9 The F2 segregation populations are numbered 1559, 1561; 1563, 1565; 1567, 1569. The groups an...

Embodiment 2

[0054] Example 2 Preliminary Mapping of S1 Gene

[0055] 1. First, scan the short arm of chromosome 6 of the three S1 near-isogenic lines (IRAT216S1-1, IRAT216S1-2, IRAT216S1-3) to find out the overlapping regions of their introgressed chromosome segments, and then use the small The population was subjected to molecular marker linkage analysis and preliminary positioning.

[0056] The specific method is as follows:

[0057] (1) Screening of S1 preliminary localization molecular markers

[0058] According to the preliminary mapping results of previous studies, the regions screened by all the markers for the preliminary mapping of S1 are mainly concentrated in the short arm of chromosome 6. Since the genome sequence of African cultivated rice has not been determined, we can only use the SSR and INDEL molecular markers between indica rice (9311) and japonica rice (Nipponbare) published on the Internet (http: / / www.gramene.org). Design a pair of molecular marker primers at int...

Embodiment 3

[0072] Example 3 S1 Gene fine mapping

[0073] This example is in S1 On the basis of the preliminary mapping of the gene, the molecular markers of the large population are used to fine-map it.

[0074] 1. Development and linkage analysis of new polymorphic molecular markers

[0075] With the continuous shrinkage of the positioning section in Example 2, it becomes more and more difficult to find the molecular markers used for further fine positioning. In the 95kb interval between RM3463 and RM19359, the present invention synthesized and detected all the SSR markers published on the Internet, but unfortunately there is no one-to-one polymorphism.

[0076]Therefore, based on the sequence alignment of the indica rice 9311 and the japonica rice Nipponbare, the inventors synthesized and detected more than a dozen pairs of INDEL markers (some of which are shown in Table 4) with differences between the two sequences segment by segment, and the result was only 2180 A pair of mark...

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Abstract

The invention discloses a cultivated rice hybrid infertility gene S1 and application thereof. The S1 gene comprises two alleles S1-j and S1-g; the nucleotide sequence of S1-j is shown as SEQ ID NO. 1; and the nucleotide sequence of S1-g is shown as SEQ ID NO. 2. A plurality of molecular markers suitable for positioning the S1 gene are firstly developed by a map-based cloning method to construct an ultra-large positioning colony suitable for positioning, the cultivated rice hybrid infertility gene S1 is positioned and cloned to obtain a candidate gene ORF12, and respective full length sequences of S1 alleles of hybrid parents are further measured, to discover that the S1 genes of the hybrid parents show allelic similarity and difference of hybrid infertility reasons. The S1 gene is a first cloned rice hybrid infertility gene at home, and can provide a firm theoretical basis for solving the problem of rice hybrid male and female sterility, guiding rice breeding and further improving the yield of rice.

Description

technical field [0001] The invention belongs to the fields of molecular plant breeding and genetic engineering. More specifically, it relates to a cultivated rice interspecific hybrid sterility gene S1 and its application. Background technique [0002] Rice is one of the most important food crops for human beings, and it is also the largest crop in my country. About half of the world's population uses rice as their main food. China's rice grain production has solved the problem of food and clothing for more than one billion people. The success of rice dwarf breeding organized by Huang Yaoxiang and others in the 1960s, and the national cooperation represented by Yuan Longping in the 1970s achieved breakthroughs in rice "three-line method" supporting seed production by using heterosis within subspecies. There have been two qualitative leaps in yield per unit area, which have played an important role in stabilizing grain production in China and the world. However, since the ...

Claims

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Application Information

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IPC IPC(8): C12N15/57C12N15/29C12Q1/68C12N15/11
Inventor 马生健
Owner LINGNAN NORMAL UNIV
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