Seedling-raising method for gracilaria algae
A technology of Gracilaria and seaweed, applied in the field of seedling cultivation of Gracilaria genus seaweed, can solve the problems of low survival rate, slow growth, small seedlings, etc., and achieve the problem of germplasm degradation, high agar content, and fast growth of seedlings Effect
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Embodiment 1
[0026] 1. Preparation for planting algae: collect mature asparagus algae (fruit sporophytes, tetraspores), select algae strains that are robust and mature, and cut off old branches with a large number of miscellaneous algae attached to the surface of the base of the algae For the algae part, the algae with a smooth surface, bright colors, no obvious attachments and necrotic tissue is used as a seed algae, washed, and the miscellaneous algae and other attached organisms are removed;
[0027] 2. Cleaning of algae: scrub the picked algae in boiled fresh water 3 times, then disinfect the algae with 3% sodium hypochlorite for 4 minutes to further remove possible residual algae and protozoa, and finally rinse in boiled fresh water Then put it in boiled sea water for later use;
[0028] 3. Drying stimulation in the shade: place the grown vegetables in a cool place with good air circulation for drying stimulation in the shade for 50 minutes, and the dehydration rate is controlled at 2...
Embodiment 2
[0039] 1. Preparation for planting algae: collect mature Gracilaria algae (carp sporophytes, tetrasporophytes), cut it into sections, take the algae sections with smooth surface, bright color, no obvious attachments and necrotic tissue as algae, Wash to remove algae and other parasites;
[0040] 2. Algae cleaning: scrub the picked algae in boiled fresh water for 3 times, then disinfect the algae with 3% sodium hypochlorite for 3 minutes to further remove possible residual algae and protozoa, and finally rinse in boiled fresh water Then put it in boiled sea water for later use;
[0041] 3. Drying stimulation in the shade: place the grown vegetables in a cool place with good air circulation for drying stimulation in the shade for 50 minutes, and the dehydration rate is controlled at 30-40%;
[0042] 4. Spore release: After the vegetables are stimulated by drying in the shade, put them in a container with sterilized seawater with a salinity of 30‰ and a glass plate at the bottom...
Embodiment 3
[0052] 1. Recovery: Take out the cultured tissue of Asparagus discus that has been stored under low light at 10°C for 1 year, replace 1 / 3 of the culture solution each time, and culture for 2 days under the light intensity of 500lux, 1000lux 2 days under the light intensity of 2500 lux, and then placed under the light intensity of 2500lux and aerated for 2 days to complete the recovery;
[0053] Add 4ppm of NO in every cubic meter of sterilized seawater in the container 3 -N and 0.4ppm PO 4 -P;
[0054] 2. Expansion of cultivation: the asparagus discoid tissue after the resuscitation is chopped with a masher, placed in the Erlenmeyer flask of sterilized seawater with a salinity of 30‰, and added 8ppm diacetate to the seawater culture solution. Germanium oxide is cultured in suspension for 10-15 days at a light intensity of 2500lux, a photoperiod of 12:12, and a temperature of 20±2°C. This step is carried out in cycles, so as to realize the expanded cultivation of germplasm; ...
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