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Method for synchronously measuring capsaicin and capsorubin in capsicum oleoresin

A technology of capsicum oleoresin and capsaicin, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low overlap rate, large difference in chemical structure of capsanthin and capsaicin, etc., so as to reduce errors and test The effect of low cost and easy operation

Inactive Publication Date: 2015-04-22
ZHONGZHOU UNIV
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AI Technical Summary

Problems solved by technology

When the absorption spectrum overlap between the components is very serious, the Y-reference method can be used, but the chemical structure of capsanthin and capsaicin is quite different, and the overlap rate is not high, so the Y-reference method cannot be used

Method used

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  • Method for synchronously measuring capsaicin and capsorubin in capsicum oleoresin
  • Method for synchronously measuring capsaicin and capsorubin in capsicum oleoresin
  • Method for synchronously measuring capsaicin and capsorubin in capsicum oleoresin

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Embodiment Construction

[0030] The present invention synchronously measures the method for capsaicin and capsanthin in capsicum oleoresin, can carry out according to the following steps:

[0031] Step 1: Determination of Capsaicin and Capsanthin in Capsicum Oleoresin Samples by Isoabsorption Point Mapping

[0032] 1) Preparation of capsicum oleoresin samples

[0033] Weigh 25g of chili powder, use 75% ethanol, solid-to-liquid ratio 1:4 (w / v), extract at 40°C for 4 hours, extract twice, combine the extracts, concentrate, and place in a blast drying oven at 40°C , dried to constant weight, obtained capsicum oleoresin sample 5.3337g;

[0034] 2) Determination of the content of capsaicin and capsanthin in capsicum oleoresin samples by means of isoabsorption point mapping method, denoted as C 1 and C 2 ;

[0035] ① Accurately weigh the capsaicin standard substance, use ethanol as a solvent, and prepare a standard solution with a concentration of 0.03 mg / ml; take the capsaicin standard substance, and u...

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Abstract

The invention provides a method for synchronously measuring capsaicin and capsorubin in a capsicum oleoresin and belongs to the technical field of detection methods of the capsicum oleoresins. According to the method for synchronously measuring the capsaicin and capsorubin in the capsicum oleoresin, an isoabsorptive point graphing method is adopted, correction is performed by virtue of reversed phase high-performance liquid chromatography, the correction coefficient is introduced, and the content of the capsaicin and capsorubin in the capsicum oleoresin can be accurately, rapidly and synchronously measured. Therefore, the defect of dual wavelength spectrophotometry is overcome, the single wavelength direct colorimetric error is reduced, and the method is particularly applicable to the fields of tracking detection and quality analysis of products in industrial production.

Description

technical field [0001] The invention belongs to the technical field of detection methods of capsicum oleoresin, and in particular relates to a method for synchronously determining capsaicin and capsanthin in capsicum oleoresin. Background technique [0002] Capsicum oleoresin is a dark red oily liquid extracted and concentrated from capsicum. It has excellent color, fragrance and taste, and is widely used as a food additive raw material. The main active ingredients of capsicum oleoresin are capsaicin and capsanthin. The demand for capsicum oleoresin at home and abroad is very large. Since it was first put on the market by Guizhou Galla Chinensis Co., Ltd. in 1997, the annual sales volume has been increasing. In 2003, the sales volume in our country was over 100 tons. In order to detect the quality of capsicum oleoresin products and determine the best production process route, it is particularly important to establish a reliable quantitative analysis method for capsicum oleo...

Claims

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Application Information

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IPC IPC(8): G01N30/89
Inventor 宋彦显闵玉涛宋淑蕴王文武李靖靖华慧颖孙于庆李建新郭林
Owner ZHONGZHOU UNIV
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