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Sphingosine monoucleosis for degrading cypermethrin and application thereof

A technology of sphingomonas and cypermethrin, which is applied in the field of biotechnology treatment of pesticide pollution, can solve the problems of high toxicity and harm to the reproductive system of rats, so as to increase added value, protect human health, and have good removal effect Effect

Active Publication Date: 2015-04-22
HENGSHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that cypermethrin has a half-life of up to 31 days in water, is highly toxic to a variety of aquatic organisms, and is also harmful to the reproductive system of rats

Method used

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  • Sphingosine monoucleosis for degrading cypermethrin and application thereof
  • Sphingosine monoucleosis for degrading cypermethrin and application thereof
  • Sphingosine monoucleosis for degrading cypermethrin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Sphingomonas WHSC-8 T the acquisition of

[0033] Sample collection: In July 2010, soil samples were collected from Hengshui Lake in Hengshui City.

[0034] Isolation and purification of bacterial strains: Weigh 5.0g of the sample and place it in a 250mL Erlenmeyer flask containing 100mL of enriched medium, add 100mg / L of cypermethrin at 30°C, 150r / min constant temperature shaker culture, transplant weekly Once, insert 10% of the inoculum into fresh medium, increase the concentration of cypermethrin in each inoculation, and increase the concentration by 100mg / L until the concentration increases by 500mg / L, and then replace the enrichment medium with basal medium , transplanted once a week, and the concentration of cypermethrin was always maintained at 500mg / L. After the hyphae grow, the strains are isolated on the ordinary medium by the plate coating method.

[0035]Preparation of separation plate: Add agar into the triangular flask containing the basic me...

Embodiment 2

[0036] Example 2: WHSC-8 T Strain biological characteristics

[0037] (1) 16S rDNA sequence similarity test (see Table 2)

[0038] Table 2 Standard strain information

[0039]

[0040] Standard strains were purchased from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen)

[0041] 16S rDNA sequence similarity: WHSC-8 T The sequence similarities with strains S1, S2, and S3 were 97.472%, 96.647%, and 96.624%, respectively. WHSC-8 T The result of DNA-DNA hybridization with S1 was 4.43%.

[0042] (2) Experimental results of polar lipids, respiratory quinones, GC content, and polyamines (see Table 3)

[0043] Polar lipids and respiratory quinone determination institution: Yunnan Institute of Microbiology, Yunnan University;

[0044] DNA (G+C) content determination agency: Guangdong Microbial Analysis and Testing Center.

[0045] Table 3 Experimental results of polar lipids, respiratory quinones, GC content, and polyamines

[0046]

[0047] (3) Determinatio...

Embodiment 3

[0055] Example 3: Strain WHSC-8 T Degradation of pesticides

[0056] (1)WHSC-8 T Bacteria preparation

[0057] WHSC-8 T Test tube bacteria inoculated in basal medium (g / L): NH 4 NO 3 1.0, MgSO 4 ·7H 2 O 0.5, (NH 4 ) 2 SO 4 0.5, KH 2 PO 4 0.5, NaCl 0.5, K 2 HPO 4 1.5, yeast extract 0.05, pH 7. In shake flasks, shaking culture to logarithmic growth phase, bacterial solution OD 600 Value is 1, add 1ml of WHSC-8 T The culture solution was centrifuged at 3000 rpm for 5 minutes, the supernatant was discarded, and then ultrapure water was added to rinse the cells twice, and the cells were prepared for pesticide degradation.

[0058] (2) Determination of strain degradation ability

[0059] Put the above bacterium into the triangular flask (g / L) filled with inorganic salt culture medium respectively according to the ratio of 5%: K 2 HPO 4 0.2, KH 2 PO 4 0.5, MgSO 4 ·7H 2 O 0.4, FeSO 4 ·7H 2 O 0.002, (NH 4 ) 2 SO 4 0.2, CaSO 4 0.08, add respectively cypermet...

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Abstract

The invention provides sphingosine monoucleosis WHSC-8T having the efficient degrading effect on cypermethrin pesticide residues and an application of the sphingosine monoucleosis. A bacterial strain is preserved in the typical culture collection center of China on November 18, 2014, and the collection serial number is CCTCC NO: M2014575. The sphingosine monoucleosis has the remarkable degrading effect on the pesticide residues of cypermethrin, phoxim, chlorpyrifos, isoprocarb, carbofuran and the like, and the rate of degrading the cypermethrin is more than 91.3%. The pesticide residue degrading bactericide has the advantages of being low in production and use cost, convenient to use, good in removing effect and suitable for being used and popularized in grain, oil and vegetable production export bases across the country or areas with pollution-free food brand marks in a large-area mode.

Description

technical field [0001] The invention belongs to the field of biotechnology treatment of pesticide pollution, and in particular relates to a sphingomonas WHSC-8 which has an efficient degradation effect on cypermethrin pesticide residues T and its application. Background technique [0002] Pyrethroid pesticides are a class of insecticides synthesized by simulating natural pyrethrins. They have the advantages of broad spectrum, high efficiency and low toxicity, and are widely used in the control of agricultural pests and sanitary pests. However, the excessive residues of pyrethroid insecticides on economic crops such as tea and vegetables have brought food safety problems. The European Union and Japan, as the two main markets for China's agricultural product exports, have continuously imposed stricter regulations on pesticide residue limits, which has put China's agricultural product exports under enormous pressure. At the same time, pyrethroid insecticides are highly toxic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A62D3/02C12R1/01A62D101/04A62D101/28
CPCA62D3/02A62D2101/04A62D2101/28C12N1/04C12N1/205C12R2001/01
Inventor 魏淑珍郭继平吴荣荣梁魁景张志强王茜王婷婷张彩凤李志涛李辉朱冰清
Owner HENGSHUI UNIVERSITY
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