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A strain of Lactobacillus plantarum and its application in active fermented milk

A technology of Lactobacillus plantarum and fermented milk, applied in the direction of Lactobacillus, application, and microbial-based methods, can solve the problems of weak acid production ability, inability to exert the health function of probiotics, texture and taste that are difficult for consumers to accept, etc. To achieve the effect of high stability performance

Active Publication Date: 2017-12-01
SHANGHAI JIAODA ONLLY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing studies have shown that Lactobacillus plantarum is used as a separate starter in dairy products. It grows extremely slowly in milk and has weak acid production ability. Its fermented milk is difficult to accept by consumers in terms of texture and taste.
Therefore, in general, Lactobacillus plantarum and Streptococcus thermophilus or combined with yogurt starter (Streptococcus thermophilus and Lactobacillus bulgaricus) are used in fermented milk products (Yu Zhihui, Zhang Xue, Li Shengyu, etc., Lactobacillus plantarum in Application in yogurt, China Dairy Industry, 2013, 41(7):27-30), but because Streptococcus thermophilus or yogurt starter grow faster in milk, the growth of Lactobacillus plantarum is inhibited, and in fermented milk products Lactobacillus plantarum has a low number of viable bacteria and cannot exert the health function of probiotics

Method used

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  • A strain of Lactobacillus plantarum and its application in active fermented milk
  • A strain of Lactobacillus plantarum and its application in active fermented milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Enzyme Activity Evaluation of Plantarum Lactobacillus LP-ONLLY

[0021] Enzyme activity is an important indicator of starter culture. The enzyme activity of Lactobacillus plantarum LP-ONLLY and laboratory-preserved strain - Lactobacillus plantarum LP6009 was semi-quantitatively analyzed using API ZYM kit (Mérieux, France). According to the operation instructions of the kit, the bacterial cells grown in the MRS culture medium were collected, and the bacterial suspension was prepared with sterile water for the next step of enzyme activity analysis. After adding bacteria and reagents, the color depth of the reagent strips is divided into 6 grades from light to dark from 0 to 5, 0 corresponds to a negative reaction, and 5 represents the strongest reaction. The approximate bacterial enzyme activity can be known from the depth of the color. Colors 1 to 5 correspond to the release of enzyme activity concentrations of 5nmoles, 10nmols, 20nmols, 30nmols and 40nmols res...

Embodiment 2

[0027] Example 2 Evaluation of milk coagulation ability of Lactobacillus plantarum LP-ONLLY

[0028] The ability to curd milk is another important property of a dairy starter. Press Lactobacillus plantarum LP-ONLLY and LP6009 by 10 7 CFU / ml was added to 10% skimmed milk powder, 10% skimmed milk powder + 0.25% yeast extract, 10% skimmed milk powder + 0.25% casein hydrolyzate, 10% skimmed milk powder + 0.25% peptone, and 10% skimmed milk powder + Incubate at 37°C in 1% glucose and evaluate the clotting time.

[0029] As can be seen from Table 2, no matter which culture medium Lactobacillus plantarum LP6009 is in, it cannot coagulate milk within 16 hours, and Lactobacillus plantarum LP-ONLLY can be curd when nitrogen sources are added (such as adding yeast extract, casein Hydrolyzate and peptone) in 10% skimmed milk powder, with rapid curdling ability.

[0030] Conclusion: Compared with ordinary strains, Lactobacillus plantarum LP-ONLLY has the ability of rapid milk coagulatio...

Embodiment 3

[0034] Example 3 Preparation of Lactobacillus plantarum LP-ONLLY freeze-dried live bacteria powder

[0035] The production strain of Lactobacillus plantarum LP-ONLLY is cultured with primary seed liquid and secondary seed liquid, then added to a fermenter for cultivation and fermentation, centrifuged and freeze-dried to make Lactobacillus plantarum LP-ONLLY bacterial powder. Lactobacillus plantarum LP-ONLLY powder suitable for the preparation of active fermented milk, the number of viable bacteria is greater than 1.0×10 11 CFU / g.

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Abstract

The invention discloses a strain of Lactobacillus plantarum and its application in active fermented milk. The Lactobacillus plantarum LP-ONLLY was preserved in the General Microorganism Center of China Microorganism Culture Collection Management Committee on December 6, 2004, and the preservation registration number is CGMCC NO.1258. The plantarum lactobacillus has high aminopeptidase activity, high β-galactosidase activity and high glucosidase activity, and its freeze-dried live bacterial powder can be applied to the preparation of active fermented milk as a separate starter. This active fermented milk not only contains high probiotic live bacteria, but also the live bacteria in it have high stability, which can supplement probiotics and exert the health function of Lactobacillus plantarum LP‑ONLLY.

Description

field of invention [0001] The invention relates to a new strain of Lactobacillus and its application in active fermented milk. More precisely, the invention describes a new strain of Lactobacillus plantarum (Lactobacillus plantarum), LP-ONLLY, CGMCC No.1258. Background technique [0002] Lactobacillus plantarum (Lactobacillus plantarum) is distributed in a variety of natural environments and is the original bacterium of the human body. In 2005, 66 cases of neonates born 1 to 7 days old in my country were taken as the research objects. The DNA PCR amplification and clonal diversity analysis of intestinal bacteria showed that Lactobacillus plantarum was the dominant lactobacillus in the intestinal tract of newborns in my country, accounting for all the tested ones. 74% of Lactobacillus (Zhang Hechun, Yang Guo, Wang Jinsong, Hang Xiaomin, etc. Research on the diversity of intestinal bacteria and Lactobacillus populations in infants. Chinese Journal of Microecology, 2005, l7(4): ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/123C12R1/25
CPCA23C9/1234C12R2001/25C12N1/205A23V2400/169
Inventor 杭晓敏张旻张和春
Owner SHANGHAI JIAODA ONLLY CO LTD
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