Pressurizing and draining device applied to DNA synthesizer

Abstract

The invention relates to a pressurizing and draining device applied to a DNA synthesizer, which comprises a synthesis baseplate, synthesis columns, a pressurizing rubber pad, a pressurizing plate, an external inert gas source and ventilating connectors, wherein the synthesis columns are arranged on the synthesis baseplate; a flange extends upwards out of the periphery of the synthesis baseplate; the flange is parallel to the synthesis columns and is higher than the synthesis columns; the pressurizing rubber pad is arranged on the flange; the pressurizing plate is sealed together with the synthesis baseplate through the pressurizing rubber pad; the ventilating connectors are arranged on the pressurizing plate and used for realizing the communication between an external inert gas source and the upper side of the synthesis columns. The pressurizing and draining device further comprises a pressurizing and draining actuating device which is used for pressurizing the pressurizing plate and the pressurizing rubber pad to seal the pressurizing rubber pad and the synthesis baseplate. Inert gas flows into the space between the upper side of the synthesis columns and the pressurizing plate via the ventilating connectors; and by controlling the pressure of the gas above the synthesis columns, the draining speed of reagents in the synthesis columns can be increased and the reaction speed of the reagents can be controlled.

Description

technical field [0001] The invention relates to a pressurized liquid discharge device, in particular to a pressurized liquid discharge device applied to a DNA synthesizer. Background technique [0002] Artificially synthesized DNA is currently the only known method for directional modification of gene sequences, and is widely used in many fields such as protein modification and life sciences, such as nucleic acid drugs, enzyme engineering, gene therapy, etc. The DNA synthesizer needs different steps such as deprotection, activation link, capping and oxidation in the synthesis process, and different reagents need to be injected into the synthesis column during the synthesis process. [0003] Different reagents in the synthesis column have different reaction speeds, and the reagents in the synthesis column need to be emptied after the reaction is completed. In order to control the reaction speed of the reagents and realize the emptying of the reagents, it is necessary to desi...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a pressurized liquid discharge device applied to a DNA synthesizer, which overcomes the defect that there is no reliable and stable liquid discharge device for the liquid discharge and cleaning links in the working process of the DNA synthesizer in the prior art

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