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Method for extracting platycodin D from platycodon grandiflorum

A technology of platycodon saponins and platycodon grandiflorum, which is applied in the field of platycodon grandiflorum extraction and separation, can solve problems such as less separation and purification of platycodon grandiflorum saponins D, and achieve the effects of less solvent damage, improved operability, and high recovery rate

Active Publication Date: 2015-04-22
SHAANXI JIAHE PHYTOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] Most of the mentions in the prior art are to extract the total saponins of Platycodon grandiflora, while the separation and purification of Platycodon grandiflora saponin D is relatively small, mainly focusing on methods such as extraction, extraction, and column chromatography.

Method used

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  • Method for extracting platycodin D from platycodon grandiflorum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Take 1 kg of Platycodon grandiflora raw material and pulverize it, use 90% ethanol aqueous solution with 5 times the mass of the raw material to extract with stirring at 80°C, and extract twice in total to obtain the extract.

[0040] The extract is separated by an ultrafiltration membrane with a molecular weight cut-off of 10,000-20,000 dalton to obtain an ultrafiltrate and a dope, and the ultra-filtrate is separated by a nanofiltration membrane with a molecular weight cut-off of 800-1000 dalton to obtain a nanofiltration and a dope, and a dope drying. Dissolve the dry matter in 8 times the amount of acetonitrile at 80°C, add activated carbon for decolorization, filter, add acetonitrile solution of 10% acetone, put it in the refrigerator to crystallize overnight, and filter with suction to obtain 3.39 g of white powdery substance, which is platycodon saponin D. The content is 98.4%, and the recovery rate is 83.4%.

Embodiment 2

[0042] Take 1 kg of Platycodon grandiflora raw material and pulverize it, and extract it with 90% ethanol aqueous solution with 5 times the mass of the raw material at 40° C. with stirring, and extract twice in total to obtain an extract.

[0043] The extract is separated by an ultrafiltration membrane with a molecular weight cut-off of 5000-8000dalton to obtain an ultrafiltrate and a dope, and the ultrafiltrate is separated by a nanofiltration membrane with a molecular weight cut-off of 400-600dalton to obtain a nanofiltration and a dope. drying. Dissolve the dry matter in 4 times the amount of 80% isopropanol at 60°C, add activated carbon for decolorization, filter, add 25% acetone in isopropanol solution, put it in the refrigerator to crystallize overnight, and filter with suction to obtain 3.53g of white powdery substance, which is Platycodon saponin D. The content is 99.5%, and the recovery rate is 87.8%.

Embodiment 3

[0045] Take 1 kg of Platycodon grandiflora raw material and pulverize it, and extract with 90% ethanol aqueous solution with 5 times the mass of the raw material at 70°C with stirring, and extract twice in total to obtain an extract.

[0046] The extract is separated by an ultrafiltration membrane with a molecular weight cut-off of 2000-5000dalton to obtain an ultrafiltrate and a dope, and the ultrafiltrate is separated by a nanofiltration membrane with a molecular weight cut-off of 150-450dalton to obtain a nanofiltrate and a dope, and a dope drying. Dissolve the dry matter with 10 times the amount of n-butanol at 100°C, add activated carbon for decolorization, filter, add acetone with 50% n-butanol solution, put it in the refrigerator to crystallize overnight, and filter with suction to obtain 3.3g of white powdery substance, which is platycodon saponin d. The yield is 0.33%, the content is 98.1%, and the recovery rate is 80.9%. Embodiment Four

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Abstract

The invention provides a method for extracting platycodin D from platycodon grandiflorum and aims to mainly solve the problems that the prior art is complex to operate, low in product content, low in recycle rate and unsuitable for large-scale production operation. The method for extracting platycodin D from the platycodon grandiflorum comprises the following steps: (1) extraction; (2) primary ultrafiltration; (3) secondary nanofiltration; and (4) recrystallization. The method for extracting platycodin D from the platycodon grandiflorum can be used for preparing a platycodin D product of which the purity is higher than 98% by just a plurality of steps, and is convenient to operate, high in product content, high in recycle rate, small in harms caused by solvents and suitable for large-scale production operation.

Description

technical field [0001] The invention belongs to the field of bellflower extraction and separation, and relates to a method for extracting bellflower saponin D, in particular to a method for extracting bellflower saponin D from bellflower. Background technique [0002] Campanulaceae (Platycodon grandiflorum (Jacq.) A.DC.) is a perennial herbaceous plant belonging to Campanulaceae. Its root is a commonly used traditional Chinese medicine, which is recorded in multiple editions of Chinese and Japanese pharmacopoeias. It has the effects of clearing the lung, relieving the throat, eliminating phlegm and discharging pus. It is used for cough with excess phlegm, chest tightness, sore throat, lung abscess and vomiting of pus. [0003] In the 1940s, Japanese scholars first began to conduct chemical research on bellflower. Studies so far have shown that in addition to polysaccharides, its main component is oleanolic acid pentacyclic triterpene saponins, and other compounds such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08
CPCC07H1/08C07H15/256C07J63/008
Inventor 王晓莹肖红张瑜郭文华肖金霞
Owner SHAANXI JIAHE PHYTOCHEM
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