Synthesis of 1-pyrenyl-carbohydrazide and application of derivative of 1-pyrenyl-carbohydrazide in specific pre-staining detection method of glycoprotein

Abstract

The invention relates to a detection technology adopting a specific fluorescence pre-staining detection method of glycoprotein, and in particular relates to synthesis of 1-pyrenyl-carbohydrazide (UGF202) and an application of a derivative of UGF202 in specific fluorescence pre-staining detection of glycoprotein. The invention also provides a method for performing specific fluorescence pre-staining detection of the glycoprotein by applying UGF202. The method comprises the following steps: adding a periodic acid solution into a protein sample to perform oxidation; adding an ascorbic acid solution to neutralize the excess periodic acid solution; adding a dye for reaction; adding a sampling buffer solution; performing electrophoresis; and observing. The pre-staining detection method has the advantages of high sensitivity, good selectivity, simple and quick operation, good reproducibility, good linear relation, good mass spectrometry compatibility, safety in use, low cost and the like. Moreover, compared with a glycoprotein staining method after gel electrophoresis, the pre-staining detection method can realize observation after electrophoresis, so that a staining step after the gel electrophoresis is saved, and the pre-staining detection method can be well applied to researches of high-flux proteomics.

Description

technical field [0001] The invention relates to a glycoprotein-specific fluorescent pre-staining detection technology, in particular to the design and synthesis of 1-pyrenyl-carbohydrazide (UGF202) and the application of its derivatives in the glycoprotein-specific fluorescent pre-staining detection method. Background technique [0002] Protein glycosylation plays a vital role in the field of life science research. As one of the most important and common post-translational modifications of proteins, at least half of mammalian proteins are known to be glycosylated. These Glycoproteins are widely distributed in tissues, cells, and body fluids, especially on the surface of cell membranes, body fluids, etc. Glycosylation plays an important role in protein folding, transportation, and positioning, and participates in receptor activation, signal transduction, etc. Guidance, cell attachment and many other important biological processes. Changes in the number of glycoproteins or ch...

AI Technical Summary

Problems solved by technology

[0004] At present, kits in the field of glycoprotein detection are mainly concentrated in post-staining, and most products are sold in the market at high value-added prices. The high price is not conducive to the development of basic biotechnology research

In particular, the cost of using existing biological reagents to carry out biological experiments in China remains high, and it is impossible to achieve the common development of economic benefits and experiments

Glycoprotein-specific fluorescent pre-staining detection technology is rarely reported

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