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Fermented Chinese herbal preparation capable of preventing escherichia coli infection in broiler chickens and application of fermented Chinese herbal preparation

A technology of fermenting Chinese herbal medicine and Escherichia coli, which is applied in the fields of application, antibacterial drugs, and medical preparations containing active ingredients, etc., can solve the problems of affecting the growth and development of broiler chickens, the loss of chicken farming, and poor results, etc., to improve the surface of the mucous membrane The balance of microorganisms or enzymes, the improvement of drug efficacy, and the effect of improving the body's immunity

Active Publication Date: 2015-04-22
山东宝来利来生物工程股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented new type of medicated food called Ferrogel (Ferritillaria) has been developed for controlling harmful enteric strains that cause illness such as chickens’ coccidiureas. These beneficial properties include improved digestion efficiency without causing damage to other organs like heart muscle tissue, reduced gastrointestive symptoms when ingested together with certain types of drugs, increased tolerancy against various pathogen agents found naturally occurring within the gut lining, reducing side reactions associated therewith, making them better suited than current methods due to their ability to activate defense systems inside the gut itself while being less effective over external sources like pesticides. Additionally, this product helps maintain an optimal level of nutrients needed throughout life because it contains fewer chemicals but still provides benefits similar to those obtained after eating fish containing these chemicals alone.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding ways to improve the health status of animals during their consumption without causing harm or even killing off beneficial substances like medicinal ingredients used in feeds.

Method used

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  • Fermented Chinese herbal preparation capable of preventing escherichia coli infection in broiler chickens and application of fermented Chinese herbal preparation
  • Fermented Chinese herbal preparation capable of preventing escherichia coli infection in broiler chickens and application of fermented Chinese herbal preparation
  • Fermented Chinese herbal preparation capable of preventing escherichia coli infection in broiler chickens and application of fermented Chinese herbal preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: the preparation of fermented Chinese herbal medicine preparation

[0035] 1 Materials and methods

[0036] 1.1 Detection of antibacterial properties of compound Chinese herbal medicine before and after fermentation

[0037] 1.1.1 Materials

[0038]Tested strains: Streptococcus suis (trade name: Streptococcus suis powder; product number: 2014041302; manufacturer: Shandong Baolai Lilai Bioengineering Co., Ltd.); Lactobacillus reuteri (commercial name: Reuteri Lactobacillus powder; product number: 2014061401; manufacturer: Shandong Baolai Lilai Bioengineering Co., Ltd.); Lactobacillus plantarum LP (preserved in the China Center for Type Culture Collection on June 21, 2010, and its preservation number is : CCTCC M 2010150, recorded in Chinese patent application CN201010240725.X); Lactobacillus rhamnosus (trade name: Lactobacillus rhamnosus bacteria powder; commodity number: 2014091203; manufacturer: Shandong Baolai Lilai Biological Engineering Co., Ltd. ); ...

Embodiment 2

[0075] Embodiment 2: compound Chinese herbal medicine safety test

[0076] (1) Acute oral toxicity test

[0077] 1 Materials and methods

[0078] 1.1 Test materials Add 20 times the volume of water (g / mL) to the compound Chinese herbal medicine, soak for 1 hour, and cook for 3 times. After filtering and merging, the combined extracts were concentrated until the volume ratio of crude drug to extract was 4:1, 2:1, 1:5, and 1:10 (g / mL), and samples were taken for gavage respectively. Mice were purchased from Shandong Lukang Pharmaceutical Co., Ltd.

[0079] 1.2 Experimental Design Take 100 mice, half male and half male, and after pre-feeding for 3 days, they are randomly divided into 5 groups. group, gavage with tap water. The intragastric dosage is 0.02mL / g mouse weight. Continuous gavage for two days, close observation for 2 hours after each gavage, regular diet after 2 hours, regular observation records every day, continuous observation for 14 days.

[0080] 1.3 Sample c...

Embodiment 3

[0132] Example 3: Anti-infection effect test of fermented Chinese herbal medicine preparation on white-feathered broiler chickens

[0133] 1 Test materials and methods

[0134] 1.1 Experimental design and diet composition

[0135] A total of 240 1-day-old broilers with uniform body weight and basically the same health status were selected and randomly divided into 4 groups, with 3 replicates in each group and 20 broilers in each replicate. During the pre-trial period of 7 days, the animals were fed with basal rations and drank boiled tap water, and the main test period was 35 days. Group I was the control group. Group II added 1% by mass of the fermented Chinese herbal preparation prepared in Example 1 of the present invention to the basal feed; Group III added 1% by mass of unfermented Chinese herbal preparation to the basal feed. The preparation method of the fermented Chinese herbal medicine preparation is the same as the preparation of the fermented Chinese herbal medici...

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Abstract

The invention discloses a fermented Chinese herbal preparation capable of preventing escherichia coli infection in broiler chickens. The active ingredient of the fermented Chinese herbal preparation is a culture obtained by fermenting the following raw material in a formula by virtue of lactobacillus in percentage by mass: 77.7-97.7% of a composite Chinese herbal medicine, 0-20% of bran, 1.3% of ammonium sulfate and 1.0% of glucose, wherein the composite Chinese herbal medicine is prepared by mixing detoxified powder of ageratina adenophora herb, bighead atractylodes rhizome, poria cocos, bunge corydalis herb, cortex phellodendri and curcuma zedoary at the mass ratio of 5:1:1:1:1:1. Furthermore, the invention also discloses a preparation method of the fermented Chinese herbal preparation. The fermented Chinese herbal preparation prepared by the invention can be used for preventing and treating escherichia coli-induced diarrhea in broiler chickens through the combination of probiotics and the Chinese herbal medicine and replacing the efficacy of antibiotic, and the fermented Chinese herbal preparation can be also used for developing the probiotic effect as a micro-ecological preparation; and the fermented Chinese herbal preparation is pure natural, free from toxicity and harm, free from drug residue, free from foreign smell, good in palatability, and safe and convenient to use.

Description

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Claims

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Application Information

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Owner 山东宝来利来生物工程股份有限公司
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