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Cultivation method of high-powdery-mildew-resistance, short-fruit-stem, and thick-flesh cucumber chromosome single segment substitution line

A single-segment substitution line, powdery mildew resistance technology, applied in the fields of botanical equipment and methods, application, plant genetic improvement, etc., can solve problems such as failure to produce, no substantial breakthrough, and slow innovation of related varieties. , to achieve the effect of short internodes, strong disease resistance and concentrated flowering period

Inactive Publication Date: 2015-04-22
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the cucumber consumption market is showing increasingly diversified characteristics. Medium and short fruit type cucumbers are more suitable for the needs of modern family structure and individualized consumption. Breakthroughs have led to slow innovation of related varieties. Therefore, for a long time, it has not been possible to produce excellent hybrids with short fruit stems, thick flesh, early maturity, and disease resistance.

Method used

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  • Cultivation method of high-powdery-mildew-resistance, short-fruit-stem, and thick-flesh cucumber chromosome single segment substitution line
  • Cultivation method of high-powdery-mildew-resistance, short-fruit-stem, and thick-flesh cucumber chromosome single segment substitution line
  • Cultivation method of high-powdery-mildew-resistance, short-fruit-stem, and thick-flesh cucumber chromosome single segment substitution line

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Breeding process such as figure 1

[0018] Parent selection process:

[0019] Female parent: D8, cited from the University of North Carolina in the United States. The stem is thick and thick. When the internodes grow to 12-14 nodes, the top is capped with a bunch of female flowers and stops growing. The leaves are dark green, the fruit is short, the flesh is thick, and the peel is dark green; tall Powdery mildew.

[0020] Male parent: JIN5-508 is a vine variety with tall plants, long internodes, long fruits, and long stems. It is separated from the domestic variety "Jinchun 5" (Tianjin Kerun Agricultural Science and Technology Co., Ltd.) for 8 generations , select long-fruit type fruits in each progeny, and obtain high resistance to cucumber powdery mildew, long-fruit, and long-fruit type inbred lines that are no longer segregated until the 8th selfing generation.

[0021] Molecular marker screening for powdery mildew resistance linkage:

[0022] The highly sus...

Embodiment 2

[0026] 1. Use D85109267 to prepare the first generation of hybrid

[0027] The hybrid combination with short fruit type (fruit length about 15 cm) female line can ensure that the F1 generation maintains the short fruit handle characteristics, and at the same time, the female rate is higher than 85%, which has great potential for increasing production.

[0028] , The basic performance of hybrids formulated with D85109267

[0029] The basic (F1) performance of the hybrid combination prepared with thornless or less thorny, all-female cucumber inbred lines as female parent and D85109267 as male parent: strong female (female rate is above 85%), strong growth potential, large leaves , thick leaves, fruit length of about 17 cm, very short or no fruit handle, no thorns, few tumors, green peel, good commerciality, resistance to powdery mildew, downy mildew and fusarium wilt, more than 15 fruits per plant , the average single fruit weight is about 115 grams, and the fruit shape is ...

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Abstract

The invention relates to a cultivation method of a high-powdery-mildew-resistance, short-fruit-stem, and thick-flesh cucumber chromosome single segment substitution line. Dwarf powdery mildew susceptible inbred line cucumbers D8 are used as a female parent, a long-bine powdery mildew resistant inbred line JIN5-508 is used as a male parent, and hybridization, backcross and molecular marker-assisted selection technology are adopted, so that a dwarf high-powdery-mildew-resistance cucumber chromosome single segment substitution line D85109267 is obtained through consecutive 12-generation backcross and 4-generation inbred selective breeding. The genetic background of the substitution line is almost identical to that of the powdery mildew susceptible female parent D8, and the only difference lies in that a chromosome single segment with length of only 0.7cM in the powdery mildew resistant male parent JIN5-508 is substituted in the genetic background. The new dwarf powdery mildew resistant cucumber line D85109267 has the advantages of short internode, concentrated flowering period, short fruit stem, thick flesh, concentrated fruit picking, high early yield and income increasing by more than 20%, and can be used for preparing hybrids for the purpose of early-maturing.

Description

technical field [0001] The invention belongs to the germplasm innovation of crops (cucumber), which is a single-segment substitution line of cucumber highly resistant to powdery mildew chromosome obtained by hybridization, backcrossing and molecular marker-assisted selection technology (the two ends of the single segment are marked by two specific SSRs) defined). The selection of this substitution line provides necessary and superior parental materials for the breeding of new cucumber varieties with high resistance to powdery mildew, short stems and thick flesh. Background technique [0002] In agricultural production, compared with conventional varieties, hybrids have the characteristics of stronger resistance and higher yield, so they often have higher cultivation benefits. About 85% of cucumber varieties are hybrids. At present, the varieties used in the production of cucumbers in my country are mainly long-fruited dense thorn cucumbers. These varieties are mainly chara...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 陈学好齐晓花许学文徐强
Owner YANGZHOU UNIV
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