Culture medium for culturing dendrobium officinale tissue cultured seedling and method for preparing culture medium
A technology of dendrobium candidum and tissue culture seedlings, which is applied in botany equipment and methods, gardening methods, gardening tools/equipment, etc., can solve the problems of low transplant survival rate of dendrobium candidum, poor sterilization reliability, and long medium disinfection time, etc. problems, achieve the effect of shortening disinfection time, improving industrial production efficiency, and shortening the cultivation cycle
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Embodiment 1
[0024] A culture medium for tissue culture seedlings of Dendrobium officinale, comprising 150 grams of mashed potatoes, 150 grams of mashed apples, 200 grams of mashed bananas, 250 grams of white sugar, 30 grams of ammonium nitrate, 30 grams of potassium nitrate, 3 grams of potassium dihydrogen phosphate, and 3 grams of boric acid , Alanine 3g, Vitamin B 1 3 grams, 0.5 grams of IBA, 0.5 grams of GA, 0.5 grams of 6-BA, 1200 ml of purified water.
[0025] The preparation method of described Dendrobium officinale tissue culture medium comprises the following steps:
[0026] S1: After peeling the potatoes, apples, and bananas, weigh them according to the above proportions, then use a grinder at a speed of 500r / min for 3 minutes, grind them evenly, and pour the three kinds of slurry into a 5L container ;
[0027] S2: White sugar, ammonium nitrate, potassium nitrate, potassium dihydrogen phosphate, boric acid, alanine, vitamin B 1 , IBA, GA, 6-BA, and purified water were put into...
Embodiment 2
[0030] A culture medium for tissue culture seedlings of Dendrobium candidum, comprising 200 grams of mashed potatoes, 200 grams of mashed apples, 250 grams of mashed bananas, 300 grams of white sugar, 30 grams of ammonium nitrate, 35 grams of potassium nitrate, 4 grams of potassium dihydrogen phosphate, and 4 grams of boric acid , Serine 4g, Vitamin B 2 5 grams, IBA0.5 grams, GA0.5 grams, 6-BA1 grams, 1350 ml of purified water.
[0031] The preparation method of described Dendrobium officinale tissue culture medium comprises the following steps:
[0032] S1: After peeling the potatoes, apples, and bananas, weigh them according to the above proportions, then use a grinder at a speed of 550r / min for 2.5 minutes, grind them evenly, and pour the three kinds of slurry into a 5L container middle;
[0033] S2: White sugar, ammonium nitrate, potassium nitrate, potassium dihydrogen phosphate, boric acid, serine, vitamin B 2 , IBA, GA, 6-BA, and purified water are put into the 5L con...
Embodiment 3
[0036] A culture medium for dendrobium candidum tissue culture seedlings, comprising 250 grams of mashed potatoes, 250 grams of apple puree, 300 grams of banana puree, 300 grams of white sugar, 35 grams of ammonium nitrate, 40 grams of potassium nitrate, 5 grams of potassium dihydrogen phosphate, and 4 grams of boric acid , Glycine 5g, Vitamin B 6 5 grams, 1 gram of IBA, 0.5 grams of GA, 1 gram of 6-BA, 1500 ml of purified water.
[0037] The preparation method of described Dendrobium officinale tissue culture medium comprises the following steps:
[0038]S1: After peeling the potatoes, apples, and bananas, weigh them according to the above ratio, and then use a grinder at a speed of 600r / min for 2 minutes to grind them evenly and pour the three kinds of slurry into a 5L container ;
[0039] S2: White sugar, ammonium nitrate, potassium nitrate, potassium dihydrogen phosphate, boric acid, glycine, vitamin B 6 , IBA, GA, 6-BA, and purified water were put into the 5L container...
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